A universal model for the secondary structure of 5.8S ribosomal RNA molecules, their contact sites with 28S ribosomal RNAs, and their prokaryotic equivalent.
A universal model for the secondary structure of 5.8S ribosomal RNA molecules, their contact sites with 28S ribosomal RNAs, and their prokaryotic equivalent.
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DOI：
10.1093/nar/12.19.7479
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发表时间：
1984-10
影响因子：
14.9
通讯作者：
J. Vaughn;S. Sperbeck;W. Ramsey;C. Lawrence
中科院分区：
文献类型：
--
作者：
J. Vaughn;S. Sperbeck;W. Ramsey;C. Lawrence
系统发育方法(参考文献2)1)被用于构建通用的5.8S rRNA二级结构模型，其中约65%的残基存在于成对结构中。保守的核苷酸主要占据未配对的区域。在五个假定的螺旋中的每一个中都证明了多个补偿性碱基的变化，从而形成了它们的证明的主要基础。5.8S rRNAs的化学和酶探测结果(参考文献)。13、32)完全符合并支持我们的模式。该模型与最近提出的5.8S rRNA模型在几个方面不同(参考文献)。3、4)，并对其进行了讨论。我们模型中的每个螺旋都被延伸到相应的细菌、叶绿体和线粒体序列，这些序列通过与真核生物的序列比对而被证明是位置保守的。这一延伸也适用于碱基配对的5.8S/28S接触点及其原核生物和细胞器对应物。这些不同分子的二级结构的一致性强烈表明，它们在原核和真核核糖体中发挥着同等的功能。
The phylogenetic approach (ref. 1) has been utilized in construction of a universal 5.8S rRNA secondary structure model, in which about 65% of the residues exist in paired structures. Conserved nucleotides primarily occupy unpaired regions. Multiple compensating base changes are demonstrated to be present in each of the five postulated helices, thereby forming a major basis for their proof. The results of chemical and enzymatic probing of 5.8S rRNAs (ref. 13, 32) are fully consistent with, and support, our model. This model differs in several ways from recently proposed 5.8S rRNA models (ref. 3, 4), which are discussed. Each of the helices in our model has been extended to the corresponding bacterial, chloroplast and mitochondrial sequences, which are demonstrated to be positionally conserved by alignment with their eukaryotic counterparts. This extension is also made for the base paired 5.8S/28S contact points, and their prokaryotic and organelle counterparts. The demonstrated identity of secondary structure in these diverse molecules strongly suggests that they perform equivalent functions in prokaryotic and eukaryotic ribosomes.