COLLAGEN CROSS-LINKING IN HUMAN-BONE AND ARTICULAR-CARTILAGE - AGE-RELATED-CHANGES IN THE CONTENT OF MATURE HYDROXYPYRIDINIUM RESIDUES
COLLAGEN CROSS-LINKING IN HUMAN-BONE AND ARTICULAR-CARTILAGE - AGE-RELATED-CHANGES IN THE CONTENT OF MATURE HYDROXYPYRIDINIUM RESIDUES
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DOI：
10.1042/bj2520495
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发表时间：
1988-06-01
影响因子：
4.1
通讯作者：
VANNESS, K
中科院分区：
文献类型：
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作者：
EYRE, DR;DICKSON, IR;VANNESS, K
羟基吡啶鎓交联氨基酸在胶原蛋白中的浓度在来自1个月至80岁的人类受试者的骨和软骨样品中测量。分别解剖和分析皮质骨和松质骨样本。在骨和软骨中，这种成熟形式的交联的含量在10-15岁时达到最大值（软骨中的量是骨中的5-10倍），然后在整个成年生活中基本上保持在相同的范围内。在骨骼中，成熟交联的两种化学变体（羟赖氨酰吡啶啉与赖氨酰吡啶啉）的比例在整个成年期都是恒定的，为3.5：1，而在软骨中，这一比例总是大于10：1。羟基吡啶鎓交联与硼氢化钠可还原的酮-胺交联的比率也随年龄而变化。骨胶原中的可还原交联在出生到25岁之间含量急剧下降，但在整个成年生活中持续存在。10-15岁时，软骨中可还原的交联几乎消失，取而代之的是羟基吡啶残留物，它们的成熟产物。松质骨和皮质骨胶原的成熟交联含量随年龄变化趋势相似，但每例受试者松质骨中的浓度始终低于皮质骨，可能反映了更高的周转率，因此松质骨的状态更不成熟。
The concentration in collagen of hydroxypyridinium cross-linking amino acids was measured in samples of bone and cartilage from human subjects aged from 1 month to 80 years. Corical and cancellous bone samples were dissected and analysed separately. In both bone and cartilage, the content of this mature form of cross-link reached a maximum by 10-15 years of age (the amount in cartilage being 5-10 times that in bone), then stayed essentially in the same range throughout adult life. In bone the ratio of the two chemical variants of the mature cross-link, hydroxylysylpyridinoline to lysylpyridinoline, was constant throughout adult life at 3.5:1, whereas in cartilage it was always greater than 10:1. The ratio of hydroxypyridinium cross-links to borohydride-reducible keto-amine cross-links also changed with age. The reducible cross-links in bone collagen decreased steeply in content between birth and 25 years, but persisted in significant amounts throughout adult life. Reducible cross-links had virtually dissappeared from cartilage by 10-15 years of age, being replaced by hydroxypyridinium residues, their maturation products. Cancellous and cortical bone collagens showed similar trends with age in their content of mature cross-links, though for each subject the concentration in cancellous bone was always lower than in cortical bone, presumably reflecting the higher turnover rate and hence the more immature state of cancellous bone.