Dopamine Modified Organic-Inorganic Hybrid Coating for Antimicrobial and Osteogenesis
Dopamine Modified Organic-Inorganic Hybrid Coating for Antimicrobial and Osteogenesis
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DOI：
10.1021/acsami.6b09457
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发表时间：
2016-12-14
影响因子：
9.5
通讯作者：
Wu, Shuilin
中科院分区：
文献类型：
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作者：
Li, Man;Liu, Xiangmei;Wu, Shuilin
采用层层组装法制备了羟基磷灰石(HA)、纳米银(NPs)和壳聚糖(CS)杂化涂层。聚多巴胺辅助(PDA辅助)涂层与HA具有良好的结合力。采用溶液法和紫外光还原法制备了银纳米粒子均匀分散在杂化涂层中。采用旋涂法制备了CS纳米薄膜，以控制杂化涂层中Ag+的释放。结果表明，3层CS涂层能有效地控制杂化涂层中Ag+的释放，PDA/HA/Ag/CS-1涂层对大肠杆菌和金黄色葡萄球菌的抑菌率分别为63.0%和51.8%。此外，两种类型的掺银涂层明显破坏了大肠杆菌的正常结构。细胞存活率测定表明，CS能有效降低杂化涂层的细胞毒性。杂化涂层在第3天和第14天表现出较高的碱性磷酸酶活性。结果表明，杂化涂层可以使钛种植体具有良好的抗菌能力以及细胞存活和成骨活性。
A hybrid coating composed of hydroxyapatite (HA), Ag nanoparticles (NPs), and chitosan (CS) was successfully prepared on a Ti substrate by a layer-by-layer assembly process. A polydopamine-assisted (PDA-assisted) coating showed a good bond with HA. Ag NPs were uniformly distributed into the hybrid coating through a solution method and ultraviolet light reduction. A CS nanofilm was deposited via spin coating to control the release of Ag+ from the hybrid coating. The results disclosed that the 3-layer CS coating could efficiently control the release of Ag+ from the hybrid coating via the Fickian diffusion mechanism and that the PDA/HA/Ag/CS-1 coating exhibited antibacterial ratios of 63.0% and 51.8% against E. coli and S. aureus, respectively. Furthermore, the normal structure of E. coli was obviously destroyed by two types of Ag doped coatings. The cell viability assay showed that CS effectively reduced the cytotoxicity of the hybrid coating after a 7 day incubation. The hybrid coating presented high ALP activities at days 3 and 14. The results reveal that hybrid coatings can endow Ti implants with good antibacterial capability as well as cell viability and osteogenic activity.