Exosome Processing and Characterization Approaches for Research and Technology Development.
Exosome Processing and Characterization Approaches for Research and Technology Development.
复制标题
DOI：
10.1002/advs.202103222
复制
发表时间：
2022-05
期刊：
影响因子：
--
通讯作者：
中科院分区：
文献类型：
--
作者：
胞外体是具有亲本细胞成分的胞外囊泡，在生物技术和生物医学研究中作为潜在的疾病生物标志物和治疗药物具有吸引力。实现这一潜力的关键是制造高质量外切体的能力；然而，与蛋白质等生物制剂不同，外切体缺乏加工和表征的标准化良好制造规范。此外，还缺乏具有良好特性的参考外切体材料来辅助选择外切体分离、纯化和分析的方法。本文通过比较外切体的加工方法和表征方法，并推荐外切体的工作流程，为外切体的研究和技术发展提供信息。本文还详细介绍了外切体的物理和化学性质、在正常生物学过程中的作用和在疾病进展中的作用，并总结了一些正在进行的临床试验。制造高质量外切体的能力对于充分发挥其作为疾病生物标志物和治疗剂的潜力至关重要。本文通过比较外切体的加工方法和表征方法，并推荐外切体的工作流程，为外切体的研究和技术发展提供信息。图中显示了不同外切体分离技术的相对产量、纯度和产量。
Exosomes are extracellular vesicles that share components of their parent cells and are attractive in biotechnology and biomedical research as potential disease biomarkers as well as therapeutic agents. Crucial to realizing this potential is the ability to manufacture high‐quality exosomes; however, unlike biologics such as proteins, exosomes lack standardized Good Manufacturing Practices for their processing and characterization. Furthermore, there is a lack of well‐characterized reference exosome materials to aid in selection of methods for exosome isolation, purification, and analysis. This review informs exosome research and technology development by comparing exosome processing and characterization methods and recommending exosome workflows. This review also provides a detailed introduction to exosomes, including their physical and chemical properties, roles in normal biological processes and in disease progression, and summarizes some of the on‐going clinical trials. The capability to manufacture high quality exosomes is essential for fully realizing their potential as disease biomarkers as well as therapeutic agents. This review informs exosome research and technology development by comparing exosome processing and characterization methods and recommending exosome workflows. The figure shows relative yield, purity, and throughput of different exosome isolation techniques.