Protection of Anthocyanin from Myrica rubra against Cerebral Ischemia-Reperfusion Injury via Modulation of the TLR4/NF-κB and NLRP3 Pathways.
Protection of Anthocyanin from Myrica rubra against Cerebral Ischemia-Reperfusion Injury via Modulation of the TLR4/NF-κB and NLRP3 Pathways.
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DOI：
10.3390/molecules23071788
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发表时间：
2018-07-20
期刊：
影响因子：
--
通讯作者：
Yuan K
中科院分区：
文献类型：
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作者：
Cui HX;Chen JH;Li JW;Cheng FR;Yuan K
杨梅含有丰富的花青素，具有良好的抗癌、抗衰老、抗氧化、抗病毒等作用。缺血性脑卒中导致的致残和死亡比例逐渐上升，成为危害人类健康的主要疾病。然而，有关花色苷对脑缺血再灌注损伤的影响的研究还很少。本研究从不同类型的猕猴桃中提取了8种花色苷提取物，并对其总花色苷和花青素-3-O-葡萄糖苷含量进行了测定。经过一周的治疗后，I/R小鼠的脑梗死体积、疾病损害程度、一氧化氮和丙二醛含量均减少，超氧化物歧化酶水平升高。结果表明，Boqi 1 MR中TA和C-3-G的含量最高（分别为22.07%和21.28%），东魁MR中PAE对小鼠脑缺血再灌注损伤具有保护作用，其机制可能与Toll样受体4（TLR 4）/核因子-κB（NF-κB）和NOD样受体pyrindomain-containing 3蛋白（NLRP 3）通路有关。
Myrica rubra (MR) is rich in anthocyanins, and it has good anti-cancer, anti-aging, antioxidant, and antiviral effects. The proportion of disability and death caused by ischemic stroke gradually increased, becoming a major disease that is harmful to human health. However, research on effects of anthocyanin from MR on cerebral ischemia-reperfusion (I/R) injury is rare. In this study, we prepared eight purified anthocyanin extracts (PAEs) from different types of MR, and examined the amounts of total anthocyanin (TA) and cyanidin-3-O-glucoside (C-3-G). After one week of PAE treatment, the cerebral infarction volume, disease damage, and contents of nitric oxide and malondialdehyde were reduced, while the level of superoxide dismutase was increased in I/R mice. Altogether, our results show that Boqi1 MR contained the most TA (22.07%) and C-3-G (21.28%), and that PAE isolated from Dongkui MR can protect the brain from I/R injury in mice, with the mechanism possibly related to the Toll-like receptor 4 (TLR4)/ nuclear factor-κB (NF-κB) and NOD-like receptor pyrin domain-containing 3 protein (NLRP3) pathways.