Germline transmission and tissue-specific expression of transgenes delivered by lentiviral vectors
Germline transmission and tissue-specific expression of transgenes delivered by lentiviral vectors
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DOI：
10.1126/science.1067081
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发表时间：
2002-02-01
期刊：
影响因子：
56.9
通讯作者：
Baltimore, D
中科院分区：
文献类型：
--
作者：
Lois, C;Hong, EJ;Baltimore, D
在体外用重组慢病毒载体感染单细胞小鼠胚胎，以产生携带由遍在表达启动子驱动的绿色荧光蛋白（GFP）基因的转基因小鼠。80%的创始小鼠携带至少一个转基因拷贝，其中90%的小鼠高水平表达GFP。后代遗传转基因并显示绿色荧光。使用具有肌肉特异性和T淋巴细胞特异性启动子的慢病毒载体产生的小鼠仅在适当的细胞类型中表达高水平的GFP。我们还产生了高水平表达GFP的转基因大鼠，这表明该技术可用于生产其他转基因动物物种。
Single-cell mouse embryos were infected in vitro with recombinant lentiviral vectors to generate transgenic mice carrying the green fluorescent protein (GFP) gene driven by a ubiquitously expressing promoter. Eighty percent of founder mice carried at least one copy of the transgene, and 90% of these expressed GFP at high levels. Progeny inherited the transgene(s) and displayed green fluorescence. Mice generated using lentiviral vectors with muscle-specific and T lymphocyte-specific promoters expressed high levels of GFP only in the appropriate cell types. We have also generated transgenic rats that express GFP at high levels, suggesting that this technique can be used to produce other transgenic animal species.