Effect of Tran on virulence through regulating metabolism and stress tolerance of Streptococcus suis serotype 2.
Effect of Tran on virulence through regulating metabolism and stress tolerance of Streptococcus suis serotype 2.
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DOI：
10.1016/j.micres.2014.03.002
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发表时间：
2014-09
影响因子：
6.7
通讯作者：
Yue Li;Jun-xi Zheng;Hui Zhang;H. Fan;Chengping Lu
中科院分区：
文献类型：
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作者：
Yue Li;Jun-xi Zheng;Hui Zhang;H. Fan;Chengping Lu
猪链球菌（SS）是一种重要的人畜共患病原体，可引起多种危及生命的感染。Tran，一种新的转录调节因子，被确定为感染相关因子inS。使用抑制减法杂交（SSH），我们组已经报道了suissertype 2。本研究构建了一个遗传突变体，与野生型ZY05719在某些生物学特性上进行比较。这表明，较长的链和相对较慢的生长可以观察到基因内缺失突变体。为了鉴定基因转录谱，进行了微阵列分析。结果表明，Tran的失活导致130个差异表达基因遍布整个基因组。其中21个基因表达上调，109个基因表达下调。大多数差异表达基因与细菌代谢有关，如磷酸转移酶系统（PTS）和热休克蛋白。在葡萄糖匮乏的情况下，平移缺失突变体的生长特性受到显著影响，同时Δtranmutant对环境胁迫高度敏感。此外，细胞粘附性下降了22.2%，斑马鱼的毒力在Δtranmutants中下降了五倍以上。这些数据证明了Tran在调节inS中的作用。瑞士2型，即通过影响细菌代谢和外部环境的应激耐受性来影响细菌的毒力。
Streptococcus suis(SS) is an important zoonotic pathogen causing a variety of life-threatening infections in pigs and humans. Tran, a novel transcriptional regulator which was identified to be an infection-related factor inS. suisserotype 2 using suppression subtractive hybridization (SSH), has been reported by our group. In this study, atrandeletion mutant was constructed to compare with the wild-type ZY05719 in some biological characteristics. It is suggested that longer chains and relatively slower growth could be observed intrandeletion mutants. In order to identify gene transcription profiles, microarray analysis was performed. It indicated that the inactivation of Tran led to 130 differentially expressed genes spread throughout the genome. Among these, 21 genes were upregulated, and 109 genes were downregulated. Most of the differentially expressed genes were involved in bacterial metabolism, such as the phosphotransferase system (PTS), and heat shock proteins. In the case of glucose scarcity, the growth characteristics oftrandeletion mutants were impacted significantly, meanwhile Δtranmutant was highly sensitive to environmental stresses. Moreover, cell adherence decreased by 22.2%, and virulence in zebrafish declined to more than five times in Δtranmutants. These data demonstrate the role of Tran in regulation inS. suisserotype 2, that is affect bacterial virulence by influencing bacterial metabolism and stress tolerance of external environment.