The use of CD 34(+) mobilized peripheral blood as a donor cell source does not improve chimerism after in utero hematopoietic stem cell transplantation in non-human primates.
The use of CD 34(+) mobilized peripheral blood as a donor cell source does not improve chimerism after in utero hematopoietic stem cell transplantation in non-human primates.
复制标题
使用CD 34( )动员的外周血作为供体细胞来源并不能改善非人灵长类动物子宫内造血干细胞移植后的嵌合状态。
DOI：
10.1111/j.1600-0684.2005.00110.x
复制
发表时间：
2005
影响因子：
0.7
通讯作者：
Andrews,RobertG
中科院分区：
文献类型：
--
作者：
Shields,LaurenceE;Gaur,Lakshmi;Delio,Patrick;Gough,Mike;Potter,Jennifer;Sieverkropp,Aimee;Andrews,RobertG
子宫内造血干细胞移植是一种治疗方法，有可能治愈许多影响免疫和造血系统的发育性疾病。在大多数胎儿造血移植的临床和实验环境中，供体细胞植入的水平一直很低，这表明即使在胎儿中，供体细胞植入也存在明显的障碍。在产后造血移植中，从动员的外周血中获得的供体细胞比从骨髓中获得的细胞更快。我们验证了一个假设，即使用从动员的外周血中获得的供体造血/干细胞可以改善非人灵长类动物子宫移植后的植入和嵌合水平。尽管从动员的外周血中使用CD34+具有潜在的竞争优势，嵌合水平与接受骨髓来源的CD34+供体细胞的一组动物没有明显的不同。基于这些结果，细胞来源的单一改变不太可能影响胎儿造血移植的临床结果。
In utero hematopoietic stem cell transplantation is a therapeutic procedure that could potentially cure many developmental diseases affecting the immune and hematopoietic systems. In most clinical and experimental settings of fetal hematopoietic transplantation the level of donor cell engraftment has been low, suggesting that even in the fetus there are significant barriers to donor cell engraftment. In postnatal hematopoietic transplantation donor cells obtained from mobilized peripheral blood engraft more rapidly than cells derived from marrow. We tested the hypothesis that use of donor hematopoietic/stem cells obtained from mobilized peripheral blood would improve engraftment and the level of chimerism after in utero transplantation in non‐human primates. Despite the potential competitive advantage from the use of CD34+from mobilized peripheral blood, the level of chimerism was not appreciably different from a group of animals receiving marrow‐derived CD34+donor cells. Based on these results, it is unlikely that this single change in cell source will influence the clinical outcome of fetal hematopoietic transplantation.