Dynamin 2 associates with complexins and is found in the acrosomal region of mammalian sperm.
Dynamin 2 associates with complexins and is found in the acrosomal region of mammalian sperm.
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Dynamin 2 与复合蛋白相关，存在于哺乳动物精子的顶体区域。
DOI：
10.1002/mrd.20660
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发表时间：
2007
影响因子：
2.5
通讯作者：
Miller,DavidJ
中科院分区：
文献类型：
--
作者：
Zhao,Longmei;Shi,Xudong;Li,Lingjun;Miller,DavidJ
先前的数据表明，复合素I是一种SNARE调节蛋白，定位于顶体和/或顶体周围，是精子顶体反应所必需的。为了了解复杂蛋白I是如何调节顶体反应的，我们使用复杂蛋白-GST下拉试验来鉴定相互作用的蛋白质。我们发现复合蛋白I和II都能与小鼠精子动力蛋白2结合。动力蛋白2是一种100 kDa的GTP酶，对许多方面的内吞作用都是必需的，但它在胞吐作用中的潜在作用尚不清楚。Dynamin 2在大鼠睾丸中有表达，在其他组织中也有广泛表达；然而，Dynamin 2在生殖细胞中的功能尚不清楚。在小鼠睾丸中检测到Dynamin 2蛋白，并在发育中的精子细胞顶体或其周围含量最丰富。此外，在哺乳动物精子顶体区域，动力素2与复合素I是共定位的。它的共定位和与复合体的相互作用我认为动力蛋白2可能在顶体形成和/或顶体胞吐过程中发挥作用。摩尔。是Reprod。戴夫。74：750-757,2007。©2006 Wiley-Liss，Inc.
Previous data showed that complexin I, a SNARE regulatory protein, is localized in and/or around the acrosome and is necessary for the acrosome reaction in sperm. To understand how complexin I regulates the acrosome reaction, we used complexin‐GST pulldown assays to identify interacting proteins. We showed that both complexins I and II bound mouse sperm dynamin 2. Dynamin 2 is a 100 kDa GTPase essential to many aspects of endocytosis but its potential role in exocytosis is unknown. Dynamin 2 is expressed in rat testis and widely expressed in other tissues; however, the function of dynamin 2 in germ cells is uncertain. Dynamin 2 protein was detected in mouse testis and was most abundant in or around the developing acrosome of spermatids. In addition, dynamin 2 was co‐localized with complexin I in the acrosomal region of mammalian sperm. Its co‐localization and interaction with complexin I suggest that dynamin 2 may play a role during acrosome formation and/or acrosomal exocytosis. Mol. Reprod. Dev. 74: 750–757, 2007. © 2006 Wiley‐Liss, Inc.