Carboxylation and dephosphorylation of phosphoenol-3-fluoropyruvate by maize leaf phosphoenolpyruvate carboxylase.
Carboxylation and dephosphorylation of phosphoenol-3-fluoropyruvate by maize leaf phosphoenolpyruvate carboxylase.
复制标题
玉米叶磷酸烯醇丙酮酸羧化酶对磷酸烯醇-3-氟丙酮酸的羧化和去磷酸化。
作者：
D. Gonzalez;C. Andreo
将类似物（Z）-磷酸烯醇-3-氟丙酮酸[（Z）-3-氟-2-（膦酰基-氧基）丙烯酸]作为玉米叶磷酸烯醇丙酮酸羧化酶的底物进行测试。用NaH 14 CO 3的研究表明，类似物被酶羧化。然而，这种反应仅占Pi释放测量的活性的十分之一。类似物的其余部分仅仅是去磷酸化的。这是第一个同时观察到羧化和去磷酸化的类似物。
The analogue (Z)-phosphoenol-3-fluoropyruvate [(Z)-3-fluoro-2-(phosphono-oxy)propenoic acid] was tested as substrate of maize leaf phosphoenolpyruvate carboxylase. Studies with NaH14CO3 indicate that the analogue is carboxylated by the enzyme. However, this reaction accounts for only one-tenth of the activity measured by Pi liberation. The rest of the analogue is merely dephosphorylated. This is the first analogue for which both carboxylation and dephosphorylation have been observed.