Array-based identification of triple-negative breast cancer cells using fluorescent nanodot-graphene oxide complexes.
Array-based identification of triple-negative breast cancer cells using fluorescent nanodot-graphene oxide complexes.
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DOI：
10.1016/j.bios.2016.03.033
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发表时间：
2016-07
影响因子：
12.6
通讯作者：
Yu Tao;D. Auguste
中科院分区：
文献类型：
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作者：
Yu Tao;D. Auguste
乳腺癌的早期和准确诊断对提高可治疗性和治愈率有很大的希望。在这里，我们报道了六种发光纳米点-氧化石墨烯复合物作为新型荧光纳米探针在传感阵列中的使用，能够有效识别健康、癌性和转移性人类乳腺细胞。该传感系统基于纳米探针-氧化石墨烯传感器元件的利用，该元件可以在乳房细胞存在时被破坏，从而提供荧光读数。利用这种多通道传感器，我们成功地鉴定了乳腺癌细胞，并区分了雌激素受体阳性、人表皮生长因子受体-2阳性和三阴性表型。该方法还允许高灵敏度（200个细胞）的细胞鉴定，具有高重复性。未知细胞样本分析表明，该传感器能够正确识别50个乳腺细胞样本中的49个，检测精度为98%。综上所述，这种基于阵列的发光纳米探针-氧化石墨烯传感平台提供了一种有用的细胞筛选工具，在生物医学诊断中具有潜在的应用前景。
Early and accurate diagnosis of breast cancer holds great promise to improve treatability and curability. Here, we report the usage of six luminescent nanodot-graphene oxide complexes as novel fluorescent nanoprobes in a sensing array capable of effectively identifying healthy, cancerous, and metastatic human breast cells. The sensory system is based on the utilization of nanoprobe-graphene oxide sensor elements that can be disrupted in the presence of breast cells to give fluorescent readouts. Using this multichannel sensor, we have successfully identified breast cancer cells and distinguished between estrogen receptor positive, human epidermal growth factor receptor-2 positive, and triple negative phenotypes. This approach also allows cell identification at high sensitivity (200 cells) with high reproducibility. The unknown cell sample analysis indicates that the sensor is able to identify 49 out of 50 breast cell samples correctly, with a detection accuracy of 98%. Taken together, this array-based luminescent nanoprobe-graphene oxide sensing platform presents a useful cell screening tool with potential applications in biomedical diagnostics.