Drosophila-Cdh1 (Rap/Fzr) a regulatory subunit of APC/C is required for synaptic morphology, synaptic transmission and locomotion.
Drosophila-Cdh1 (Rap/Fzr) a regulatory subunit of APC/C is required for synaptic morphology, synaptic transmission and locomotion.
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DOI：
10.1016/j.ijdevneu.2013.07.002
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发表时间：
2013-11
期刊：
影响因子：
--
通讯作者：
Venkatesh T
中科院分区：
文献类型：
--
作者：
Wise A;Schatoff E;Flores J;Hua SY;Ueda A;Wu CF;Venkatesh T
The assembly of functional synapses requires the orchestration of the synthesis and degradation of a multitude of proteins. Protein degradation and modification by the conserved ubiquitination pathway has emerged as a key cellular regulatory mechanism during nervous system development and function. The anaphase promoting complex/cyclosome (APC/C) is a multi-subunit ubiquitin ligase complex primarily characterized for its role in the regulation of mitosis. In recent years, a role for APC/C in nervous system development and function has been rapidly emerging. In the mammalian central nervous system the activator subunit, APC/C-Cdh1, has been shown to be a regulator of axon growth and dendrite morphogenesis. In the Drosophila peripheral nervous system (PNS), APC2, a ligase subunit of the APC/C complex has been shown to regulate synaptic bouton size and activity. To investigate the role of APC/C-Cdh1 at the synapse we examined loss-of-function mutants of Rap/Fzr (Retina aberrant in pattern/Fizzy related), a Drosophila homolog of the mammalian Cdh1 during the development of the larval neuromuscular junction in Drosophila. Our cell biological, ultrastructural, electrophysiological, and behavioral data showed that rap/fzr loss-of-function mutations lead to changes in synaptic structure and function as well as locomotion defects. Data presented here show changes in size and morphology of synaptic boutons, and, muscle tissue organization. Electrophysiological experiments show that loss-of-function mutants exhibit increased frequency of spontaneous miniature synaptic potentials, indicating a higher rate of spontaneous synaptic vesicle fusion events. In addition, larval locomotion and peristaltic movement were also impaired. These findings suggest a role for Drosophila APC/C-Cdh1 mediated ubiquitination in regulating synaptic morphology, function and integrity of muscle structure in the peripheral nervous system.
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影响因子：
56.9
作者：
Konishi, Y;Stegm端ller, J;Bonni, A
通讯作者：
Bonni, A
影响因子：
1.9
作者：
Feng, YF;Ueda, A;Wu, CF
通讯作者：
Wu, CF
影响因子：
9.2
作者：
Jacobs, HW;Richter, DO;Lehner, CF
通讯作者：
Lehner, CF
影响因子：
3.3
作者：
Kaplow, Margarita E.;Korayem, Adam H.;Venkatesh, Tadmiri R.
通讯作者：
Venkatesh, Tadmiri R.
DOI：
10.1073/pnas.96.20.11317
发表时间：
1999-09-28
影响因子：
11.1
作者：
Gieffers, C;Peters, BH;Peters, JM
通讯作者：
Peters, JM