Platinum nanoparticle-facilitated reflective surfaces for non-contact temperature control in microfluidic devices for PCR amplification.
Platinum nanoparticle-facilitated reflective surfaces for non-contact temperature control in microfluidic devices for PCR amplification.
复制标题
铂纳米颗粒促进的反射表面，用于 PCR 扩增微流体装置中的非接触式温度控制。
DOI：
10.1039/c1lc20779b
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发表时间：
2012
期刊：
影响因子：
6.1
通讯作者：
Landers,JamesP
中科院分区：
文献类型：
--
作者：
Leslie,DanielC;Seker,Erkin;Bazydlo,LindsayAL;Strachan,BrionyC;Landers,JamesP
聚合酶链反应（PCR）对于具有临床、生物学或法医学相关性的DNA或RNA的靶序列的扩增至关重要。虽然非本征法布里-珀罗干涉测量法（EFPI）已被证明足以用于非接触式温度感测，但难以限定半反射、对于PCR相容性无反应性且对于热结合具有粘附性的反射表面，这限制了其开发。通过在微流体腔室的表面上并入使用铂纳米颗粒单层的热驱动自组装制造的反射表面，产生增强的EFPI信号，从而允许使用红外介导加热、对流强制空气冷却和干涉温度感测的非接触式微流体温度控制仪器。干涉仪最初在PCR室中使用微型铜-康铜热电偶进行校准，根据室深度，温度灵敏度为−22.0至−32.8 nm· °C−1。这种通用校准可以在任意尺寸的任何设备中实现精确的温度控制，从而实现各种应用的通用性。独特的是，这种用于PCR热循环的非接触式温度控制被应用于用于人类遗传分析的STR基因座的扩增，其中使用基于EFPI的非接触式热循环成功地扩增了9个STR基因座用于人类鉴定。
The polymerase chain reaction (PCR) is critical for amplification of target sequences of DNA or RNA that have clinical, biological or forensic relevance. While extrinsic Fabry-Perot interferometry (EFPI) has been shown to be adequate for non-contact temperature sensing, the difficulty in defining a reflective surface that is semi-reflective, non-reactive for PCR compatibility and adherent for thermal bonding has limited its exploitation. Through the incorporation of a reflective surface fabricated using a thermally driven self-assembly of a platinum nanoparticle monolayer on the surface of the microfluidic chamber, an enhanced EFPI signal results, allowing for non-contact microfluidic temperature control instrumentation that uses infrared-mediated heating, convective forced-air cooling, and interferometic temperature sensing. The interferometer is originally calibrated with a miniature copper-constantan thermocouple in the PCR chamber resulting in temperature sensitivities of −22.0 to −32.8 nm· °C−1, depending on the chamber depth. This universal calibration enables accurate temperature control in any device with arbitrary dimensions, thereby allowing versatility in various applications. Uniquely, this non-contact temperature control for PCR thermocycling is applied to the amplification of STR loci for human genetic profiling, where nine STR loci are successfully amplified for human identification using the EFPI-based non-contact thermocycling.