DIFFERENTIATION OF CD3-4-8- HUMAN FETAL THYMOCYTES INVIVO - CHARACTERIZATION OF A CD3-4+8- INTERMEDIATE
DIFFERENTIATION OF CD3-4-8- HUMAN FETAL THYMOCYTES INVIVO - CHARACTERIZATION OF A CD3-4+8- INTERMEDIATE
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DOI：
10.1084/jem.178.1.265
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发表时间：
1993-07-01
影响因子：
15.3
通讯作者：
WALLER, EK
中科院分区：
文献类型：
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作者：
KRAFT, DL;WEISSMAN, IL;WALLER, EK
通过将胎儿胸腺祖细胞群体注射到SCID-hu小鼠的人胸腺异种移植物中来检查人胸腺细胞分化。用亲脂性染料PKH 2荧光标记胸腺祖细胞。其后代的表型可以通过具有非常高的荧光PKH 2信号的细胞的流式细胞术分析来鉴定。胸腺内注射纯化的三阴性（TN）CD 3 -4-8-胸腺细胞导致CD 3 -4+8-、CD 3 -4+8+和CD 3 +4+8+细胞的顺序出现，随后在4天内出现少量表型成熟的CD 3 +4+8-和CD 3 +4-8+细胞。经胸腺内注射的分选的CD 3 -4+8-胸腺细胞迅速分化为CD 4 +8+细胞。在细胞周期中，CD 4 +8+胎儿胸腺细胞分化为表型成熟的CD 3 +4+8-和CD 3 +4-8+细胞，而未分化的CD 4 +8+细胞经胸腺内移植后不能分化。基于PKH 2标记强度的降低，估计在不同时间点注射TN胸腺细胞及其后代之间发生的细胞分裂数。TN群体的细胞周期的平均长度计算为24小时。因此，SCID-hu模型提供了一个有用的工具，研究在体内的人胸腺细胞的细胞分裂和分化的动力学。
Human thymocyte differentiation was examined by injecting fetal thymic progenitor populations into human thymic xenografts in SCID-hu mice. Thymic progenitors were fluorescently labeled with the lipophilic dye PKH2. The phenotypes of their progeny could be identified by flow cytometric analysis of cells with a very high fluorescent PKH2 signal. Intrathymic injection of purified triple negative (TN) CD3-4-8- thymocytes resulted in the sequential appearance of CD3-4+8-, CD3-4+8+, and CD3+4+8+ cells, with the subsequent appearance of small numbers of phenotypically mature CD3+4+8- and CD3+4-8+ cells over a 4-d period. Sorted CD3-4+8- thymocytes injected intrathymically rapidly differentiated to CD4+8+ cells. CD4+8+ fetal thymocytes in cell cycle differentiated into phenotypically mature CD3+4+8- and CD3+4-8+ populations, whereas nondividing CD4+8+ cells failed to differentiate after intrathymic transfer. The number of cell divisions that occurred between the injection of TN thymocytes and their progeny at different time points was estimated based on the decrease in the intensity of the PKH2 label. The average length of the cell cycle for the TN population was calculated to be 24 h. The SCID-hu model thus provides a useful tool for studying the kinetics of cell division and differentiation of human thymocytes in vivo.