Identification of Cd-responsive RNA helicase genes and expression of a putative BnRH 24 mediated by miR158 in canola (Brassica napus)
Identification of Cd-responsive RNA helicase genes and expression of a putative BnRH 24 mediated by miR158 in canola (Brassica napus)
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油菜（甘蓝型油菜）中镉响应性 RNA 解旋酶基因的鉴定以及 miR158 介导的推定 BnRH 24 的表达
DOI：
10.1016/j.ecoenv.2018.03.081
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发表时间：
2018
影响因子：
6.8
通讯作者：
Yang Zhi Min
中科院分区：
文献类型：
--
作者：
Zhang Xian Duo;Sun Jia Yun;You Yuan Yuan;Song Jian Bo;Yang Zhi Min
RNA解旋酶在RNA剪接、转运、编辑和降解、蛋白质翻译起始和siRNA介导的基因沉默中起着至关重要的作用。然而，关于它们在油菜（Brassica napus）中的功能性的知识是罕见的。在本研究中，我们鉴定并注释了271个B.利用生物信息学和高通量RNA测序（RNA-seq）。已确定了DEAD-box、DEAH-box或DExD/H-box三个亚家族。通过RNA-seq确认了195种RNA解旋酶，并且鉴定了49种对镉（Cd）胁迫的差异响应（> 1.5倍变化，p <0.05）。作为一个例子，我们在Cd胁迫下功能性地鉴定了编码aBnRH 24的BnaA 04 g26450 D，BnRH 24是一个在整个生命周期中表达的组成型基因。使用我们先前生成的degradome数据集，我们发现BnRH 24可以被miR 158切割，这表明BnRH 24是B中miR 158的靶点。油菜。成熟的miR 158在B.镉胁迫下油菜的生长状况。B. napusmiR 158和BnRH 24在正常条件下的转录后水平表达，Cd支持miR 158对BnRH 24的转录后调节。BnRH 24在拟南芥中的异位表达表明，转基因植株对Cd的毒性表现出更高的敏感性，表现为根伸长、鲜重产量、叶绿素积累和O2-等氧化产物的增加。H2 O2和硫代巴比妥酸反应性物质（TBARS），表明miR 158对BnRH 24水平的调控可能是植物对Cd的耐受性所必需的。
RNA helicases play crucial roles in RNA splicing, transport, editing and degradation, protein translation initiation and siRNA-mediated gene silencing. However, knowledge about their functionality in rapeseed (Brassica napus) is rare. In the study, we identified and annotated 271 RNA helicase genes fromB. napususing bioinformatics and high-throughput RNA-sequencing (RNA-seq). Three subfamilies DEAD-box, DEAH-box, or DExD/H-box have been identified. One hundred and ninety-five RNA helicases were confirmed by RNA-seq and 49 were identified to differentially respond to cadmium (Cd) stress (> 1.5 fold change,p <0.05). As an example, we functionally specifiedBnaA04g26450Dencoding aBnRH24under Cd exposure.BnRH24is a constitutive gene expressing throughout the life span. Using our previously generated degradome datasets, we found thatBnRH24can be cleaved by miR158, suggesting thatBnRH24is a target of miR158 inB. napus. The mature miR158 was induced, whileBnRH24was repressed inB. napusunder Cd stress. The contrasting expression pattern ofB. napusmiR158 andBnRH24under the normal and Cd would support the post-transcriptional regulation ofBnRH24by miR158. Ectopic expression ofBnRH24in Arabidopsis revealed that the transgenic lines showed more sensitivity to Cd toxicity by reducing root elongation, fresh mass production, chlorophyll accumulation and increasing oxidative products such as O2-., H2O2and thiobarbituric acid reactive substances (TBARS), indicating that the controlling the level ofBnRH24by miR158 may be required for Cd tolerance in plants.