The antibacterial and antibiofilm efficacies of a liposomal peptide originating from rice bran protein against Listeria monocytogenes
The antibacterial and antibiofilm efficacies of a liposomal peptide originating from rice bran protein against Listeria monocytogenes
复制标题
米糠蛋白脂质体肽对单核细胞增生李斯特氏菌的抗菌和抗生物膜功效
DOI：
10.1039/c7fo00994a
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发表时间：
2017-11-01
期刊：
影响因子：
6.1
通讯作者：
Tang, Wenting
中科院分区：
文献类型：
--
作者：
Pu, Chuanfen;Tang, Wenting
为探索一种来源于食品的天然抗菌肽，从米糠蛋白菠萝蛋白酶水解液中分离纯化了一种抗菌肽Alpep 7。通过超高效液相色谱串联基质辅助激光解吸/电离四极杆飞行时间质谱法（MALDI Q-TOF MS）将所得氨基酸产物鉴定为KVDHFPL（Lys-Val-Asp-His-Phe-Pro-Leu）。此外，为了评估将肽的脂质体包封靶向递送至李斯特菌生物膜的可能性，进一步由摩尔比为10：3：2的二棕榈酰磷脂酰胆碱、硬脂胺和胆固醇的混合物制备负载Alpep 7的脂质体，并通过分析粒度、ζ电位、微观形貌和储存稳定性来表征。结果表明，脂质体呈球形，平均粒径小于200 nm。脂质体在4 ℃下储存4周后保持有利的稳定性。通过微量肉汤稀释、再生长分析和共聚焦扫描激光显微镜对游离和脂质体Alpep 7的活性进行比较，表明脂质体递送在脂质体初始暴露于生物膜期间更有效。热力学分析表明，脂质体Alpep 7吸附到生物膜上是一个自发的放热过程。这些结果可能为治疗细菌污染提供一种自然的手段，并指导脂质体在食品工业中将抗菌剂靶向递送到致病生物膜中的潜在应用。
With the aim of exploring a natural antilisterial peptide from food-derived origin, an antibacterial peptide named as Alpep7 was purified from the bromelain hydrolysate of rice bran protein (RBP) in this study. The resulting amino acid consequence was identified as KVDHFPL (Lys-Val-Asp-His-Phe-Pro-Leu) by ultra-performance liquid chromatography tandem matrix-assisted laser desorption/ionisation quadrupole time-of-flight mass spectrometry (MALDI Q-TOF MS). In addition, to assess the probability of the targeted delivery of liposome encapsulation of the peptide to Listeria biofilm, Alpep7-loaded liposomes were further prepared from a mixture of dipalmitoylphosphatidylcholine, stearylamine and cholesterol in a molar ratio of 10 : 3 : 2 and characterised by the analysis of particle size, zeta potential, microtopography and storage stability. The results showed that the liposomes exhibited a well-defined spherical shape, with an average diameter below 200 nm. The liposomes maintained favourable stability after storage at 4 degrees C for 4 weeks. Comparisons between the activities of free and liposomal Alpep7 via microbroth dilution, regrowth analysis and confocal scanning laser microscopy suggested that liposomal delivery was more effective during the initial exposure of the liposomes to the biofilms. The thermodynamic analysis indicated that the adsorption of liposomal Alpep7 to the listerial biofilm was a spontaneous, exothermic process. The results may provide a natural means for the treatment of listerial contamination and guide the potential application of liposomes for the targeted delivery of antimicrobials to pathogenic biofilms in the food industry.