Application of a Novel Method for Subsequent Evaluation of Sinusoids and Postsinusoidal Venules after Ischemia-Reperfusion Injury of Rat Liver
Application of a Novel Method for Subsequent Evaluation of Sinusoids and Postsinusoidal Venules after Ischemia-Reperfusion Injury of Rat Liver
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大鼠肝脏缺血再灌注损伤后正弦波和窦后微静脉后续评估的新方法的应用
DOI：
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发表时间：
1998
影响因子：
1.6
通讯作者：
K. Messmer
中科院分区：
文献类型：
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作者：
T. Kondo;S. Okamoto;T. Todoroki;T. Hirano;F. Schildberg;K. Messmer
尽管几项活体荧光显微镜研究表明，肝脏缺血再灌注期间会诱导微循环紊乱，但这些数据是随机选择微血管区域和微血管获得的。对相同微血管的重复观察尚未进行。使用特殊设计的盖板玻璃，现在可以在整个再灌注时间内反复重新定位所需的观察部位。本研究旨在探讨再灌注时间对肝脏微血管灌注状态的影响。与基线值（98.0±0.3%）相比，缺血20分钟可显著降低血流灌注率（29.1±10.2%）。在再灌注30min、60min和120min时，灌注的窦状体百分比恢复到62.8±6.6、67.5±5.7和77.2±5.4%。同一窦状窦内停滞白细胞数基线时为6.2±1.9个/小叶，再灌注120 min时增加到22.3±3.6个/小叶。缺血前窦后小静脉内粘附的白细胞数为53.5±12.5/mm2，再灌注120 min时增至414.2±62.5/mm2。我们已经证明，在120分钟的再灌注期间，随着最初严重抑制的窦状静脉灌注的衰减，窦状静脉和静脉的白细胞粘附均稳步增加。在再灌注早期和随后的再灌注阶段证实了无回流现象。
Although several intravital fluorescence microscopic studies demonstrated that microcirculatory derangement is induced during liver ischemia-reperfusion, these data were obtained from randomly selected microvascular areas and microvessels. Repeated observation of the identical microvessels has not been performed yet. Using a specially designed cover glass, it is now possible to relocate desired sites of observation repeatedly over the whole reperfusion time. The aim of this study was to determine the impact of reperfusion time on hepatic microvascular perfusion state. Twenty minutes of ischemia induced a significant decrease in sinusoidal perfusion rate (29.1 ± 10.2%) as compared with baseline values (98.0 ± 0.3%). At 30, 60, and 120 min of reperfusion, the percentage of perfused sinusoids recovered to 62.8 ± 6.6, 67.5 ± 5.7, and 77.2 ± 5.4%. The number of stagnant leukocytes in the same sinusoids was 6.2 ± 1.9/lobule at baseline and increased to 22.3 ± 3.6/lobule at 120 min of reperfusion. The number of leukocytes adhering within postsinusoidal venules was 53.5 ± 12.5/mm2 before ischemia and increased to 414.2 ± 62.5/mm2 at 120 min of reperfusion. We have demonstrated that during 120 min of reperfusion, there was a steady increase in both sinusoidal and venular leukocyte adhesion along with an attenuation of the initially severely depressed sinusoidal perfusion. A no-reflow phenomenon at an early phase of reperfusion and subsequent reflow were proven.
肝缺血再灌注过程中白细胞积累与微血管损伤之间的空间关系。
DOI：
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发表时间：
1993
期刊：
International journal of microcirculation, clinical and experimental
影响因子：
--
作者：
Ferguson,D;McDonagh,PF;Biewer,J;Paidas,CN;Clemens,MG
通讯作者：
Clemens,MG