Functional genomics and cancer drug target discovery.
Functional genomics and cancer drug target discovery.
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发表时间：
2010-06
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通讯作者：
S. Moody;J. Boehm;D. Barbie;W. Hahn
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作者：
S. Moody;J. Boehm;D. Barbie;W. Hahn
全基因组测序、拷贝数分析和表达谱分析技术的最新发展使得能够产生对癌症基因组的全面描述。然而，尽管肿瘤和癌细胞系的结构分析和表达谱可以允许鉴定在恶性状态下改变的候选分子，但功能分析对于确认这些基因为癌基因或肿瘤抑制基因是必要的。此外，最近的研究表明，肿瘤细胞也依赖于合成的致命靶点，这些靶点在癌症基因组中不会突变或扩增;功能基因组学筛选可以促进此类靶点的发现。这篇综述概述了功能基因组学研究的工具，并讨论了最近的研究，涉及使用这些工具来确定潜在的新的药物靶点在癌症。
The recent development of technologies for whole-genome sequencing, copy number analysis and expression profiling enables the generation of comprehensive descriptions of cancer genomes. However, although the structural analysis and expression profiling of tumors and cancer cell lines can allow the identification of candidate molecules that are altered in the malignant state, functional analyses are necessary to confirm such genes as oncogenes or tumor suppressors. Moreover, recent research suggests that tumor cells also depend on synthetic lethal targets, which are not mutated or amplified in cancer genomes; functional genomics screening can facilitate the discovery of such targets. This review provides an overview of the tools available for the study of functional genomics, and discusses recent research involving the use of these tools to identify potential novel drug targets in cancer.