Fluorescent silica nanoparticles modified chemically with terbium complexes as potential bioimaging probes: their fluorescence and colloidal properties in water
Fluorescent silica nanoparticles modified chemically with terbium complexes as potential bioimaging probes: their fluorescence and colloidal properties in water
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DOI：
10.1039/c4nj01222d
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发表时间：
2015-02
影响因子：
3.3
通讯作者：
Yoshio Nakahara;Yoichi Tatsumi;I. Akimoto;Shusuke Osaki;Motomichi Doi;K. Kimura
中科院分区：
文献类型：
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作者：
Yoshio Nakahara;Yoichi Tatsumi;I. Akimoto;Shusuke Osaki;Motomichi Doi;K. Kimura
硅基化荧光镧系配合物的合成通常需要复杂的反应方案。本研究通过在含甲酰基的铽配合物与3-氨基丙基三乙氧基硅烷之间形成席夫碱，用非常简单的方法制备了硅化铽配合物。以硅基化铽配合物为原料，以Triton X-100为表面活性剂，在环己烷中采用反胶束法制备了高荧光的铽配合物修饰二氧化硅纳米颗粒（Tb-SNPs）。与游离铽配合物相比，Tb-SNPs的荧光特性在光稳定性、pH依赖性和荧光寿命方面得到了显著改善。此外，Tb-SNPs在不同盐浓度和ph的水溶液条件下几乎没有聚集。从这些结果可以看出，Tb-SNPs在生理水条件下是适用的。此外，作为应用模型，tb - snp被用作非洲绿猴肾细胞成像的荧光标记。
It generally requires a complicated reaction protocol for the synthesis of silylated fluorescent lanthanide complexes. In this study, a silylated terbium complex was prepared by a very simple procedure through formation of a Schiff base between a terbium complex bearing a formyl group and 3-aminopropyltriethoxysilane. Using the silylated terbium complex, highly fluorescent silica nanoparticles modified chemically with terbium complexes (Tb-SNPs) were efficiently synthesized by a reverse micelle method with Triton X-100 as a surfactant in cyclohexane. The fluorescence properties of Tb-SNPs were remarkably improved by photostability, pH dependence and fluorescence lifetime, compared to free terbium complexes. Also, Tb-SNPs hardly aggregated under aqueous conditions with different salt concentrations and pHs. From these results, it was found that Tb-SNPs are applicable under physiological aqueous conditions. Furthermore, as an application model, Tb-SNPs were used as the fluorescent label for the imaging of African green monkey kidney cells.