Anesthesia and surgery induce cognitive dysfunction in elderly male mice: the role of gut microbiota
Anesthesia and surgery induce cognitive dysfunction in elderly male mice: the role of gut microbiota
复制标题
麻醉和手术导致老年雄性小鼠认知功能障碍：肠道微生物群的作用
DOI：
10.18632/aging.101871
复制
发表时间：
2019-03-31
期刊：
影响因子：
5.2
通讯作者：
Luo, Ailin
中科院分区：
文献类型：
--
作者：
Zhan, Gaofeng;Hua, Dongyu;Luo, Ailin
众所周知，老年患者术后认知功能障碍（POCD）的发生率较高。然而，POCD的发病机制和治疗机制尚未完全阐明。肠道微生物群的影响，特别是在调节大脑功能方面，逐渐引起了越来越多的关注。在这项研究中，我们研究了肠道微生物群在老年雄性小鼠POCD中的潜在作用，并试图确定肠道微生物群的改变是否有助于POCD的诊断。通过行为学结果的层次聚类分析将POCD和非POCD小鼠分类。此外，肠道微生物群的α-和β-多样性在各组之间显示出差异。POCD小鼠与非POCD小鼠相比，共有24种肠道细菌发生显著变化，其中13种肠道细菌与Morris水迷宫试验（MWMT）中的逃避潜伏期显著相关。值得注意的是，受试者工作特征曲线显示，脱盐杆菌科和脱盐杆菌属是诊断POCD的潜在重要细菌。这些发现表明，肠道菌群组成的改变可能参与老年小鼠POCD的发病机制。调节特定肠道细菌的新治疗策略可能有助于预防和治疗POCD。
It is well known that the incidence of postoperative cognitive dysfunction (POCD) is high in elderly patients. The pathogenesis and therapeutic mechanisms of POCD, however, have not yet been completely elucidated. The effects of gut microbiota, particularly in terms of regulating brain function, have gradually attracted increasing attention. In this study, we investigated the potential role of gut microbiota in POCD in aged male mice and attempted to determine whether alterations in gut microbiota would be helpful in the diagnosis of POCD. POCD and non-POCD mice were classified by hierarchical cluster analysis of behavioral results. Additionally, α- and β-diversity of gut microbiota showed a differential profile between the groups. In total, 24 gut bacteria were significantly altered in POCD mice compared with those in non-POCD mice, in which 13 gut bacteria were significantly correlated with escape latency in the Morris water maze test (MWMT). Remarkably, receiver operating characteristic curves revealed that the Dehalobacteriaceae family and Dehalobacterium genus are potentially important bacteria for the diagnosis of POCD. These findings indicate that alterations in the composition of gut microbiota are probably involved in the pathogenesis of POCD in aged mice. Novel therapeutic strategies regulating specific gut bacteria may be helpful for the prevention and treatment of POCD.