EFFECTS OF HUMAN INTERFERON ON CELLULAR-RESPONSE TO UV IN UV-SENSITIVE HUMAN CELL STRAINS
EFFECTS OF HUMAN INTERFERON ON CELLULAR-RESPONSE TO UV IN UV-SENSITIVE HUMAN CELL STRAINS
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DOI：
10.1016/0027-5107(88)90056-5
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发表时间：
1988-03-01
期刊：
影响因子：
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通讯作者：
OKAMOTO, S
中科院分区：
文献类型：
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作者：
SUZUKI, N;SUZUKI, H;OKAMOTO, S
人RSa细胞系的细胞，具有高敏感性的紫外线杀伤和低能力的DNA修复，当预处理1-100单位/ml的人干扰素（HuIFN）制剂超过12小时前照射，获得了增强的紫外线诱导的DNA修复复制合成与总细胞DNA合成和紫外线存活的抑制恢复。提示和短暂的诱导纤溶酶原激活剂的活性也被发现在5分钟内紫外线照射后，在细胞预处理与HuIFN，但不是在细胞非预处理与HuIFN。观察到HuIFN的增强和诱导作用，与所用HuIFN制剂的种类无关（α，β。或γ，和天然或重组的）和来源于Cockayne综合征和着色性干皮病成纤维细胞（XP 1 KY）的其它UV敏感性成纤维细胞。然而，所有的DNA修复合成的增强和诱导的纤溶酶原激活剂活性的HuIFN被抑制治疗后立即用放线菌酮UV照射。
Cells of a human RSa cell line, with high sensitivity to UV killing and low capacity for DNA repair, when pretreated with 1-100 units/ml of human interferon (HuIFN) preparations for more than 12 h before irradiation, acquired an enhancement of UV-induced DNA-repair replication synthesis in association with recovery from inhibition of total cellular DNA synthesis and UV survival. Prompt and transient induction of plasminogen activator activities was also found within 5 min after UV irradiation in the cells pretreated with HuIFN but not in the cells non-pretreated with HuIFN. The enhancement and induction effects of HuIFN were observed, irrespective of the kind of HuIFN preparation used (.alpha., .beta. or .gamma., and natural or recombinant) and in other UV-sensitive fibroblast cells which were derived from Cockayne syndrome and xeroderma pigmentosum fibroblasts (XP1KY). However, all of the enhancement of DNA-repair synthesis and the induction of plasminogen activator activities by HuIFN was suppressed by treatment with cycloheximide immediately after UV irradiation.