Macromolecule biosynthesis: a key function of sleep
Macromolecule biosynthesis: a key function of sleep
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DOI：
10.1152/physiolgenomics.00275.2006
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发表时间：
2007-11-14
影响因子：
4.6
通讯作者：
Pack, Allan I.
中科院分区：
文献类型：
--
作者：
Mackiewicz, Miroslaw;Shockley, Keith R.;Pack, Allan I.
睡眠的功能仍然是生物学中一个未回答的主要问题。我们评估了不同睡眠时间和睡眠剥夺时间后小鼠大脑皮层和下丘脑基因表达的变化。行为状态之间的基因表达存在显着差异;我们确定了大脑皮层中的3，988个基因和下丘脑中的823个基因，这些基因在睡眠和睡眠剥夺之间的表达模式发生了变化。在睡眠期间，各种基因转录物的稳态水平的变化非常常见，因为大脑皮层中的2，090个基因和下丘脑中的409个基因被定义为睡眠特异性的，并且在睡眠期间改变（增加或减少）它们的表达。最大类别的过度表达的基因增加表达与睡眠是那些参与生物合成和运输。在大脑皮层和下丘脑中，在睡眠期间，编码各种胆固醇合成酶的多个基因以及脂质运输蛋白质的表达上调。在睡眠过程中，参与蛋白质、血红素合成和维持囊泡池的基因，以及抗氧化酶和编码能量调节途径蛋白质的基因也上调。我们假设，在睡眠期间，有一个多个关键细胞成分的重建，为随后的觉醒做准备。
The function(s) of sleep remains a major unanswered question in biology. We assessed changes in gene expression in the mouse cerebral cortex and hypothalamus following different durations of sleep and periods of sleep deprivation. There were significant differences in gene expression between behavioral states; we identified 3,988 genes in the cerebral cortex and 823 genes in the hypothalamus with altered expression patterns between sleep and sleep deprivation. Changes in the steady-state level of transcripts for various genes are remarkably common during sleep, as 2,090 genes in the cerebral cortex and 409 genes in the hypothalamus were defined as sleep specific and changed (increased or decreased) their expression during sleep. The largest categories of overrepresented genes increasing expression with sleep were those involved in biosynthesis and transport. In both the cerebral cortex and hypothalamus, during sleep there was upregulation of multiple genes encoding various enzymes involved in cholesterol synthesis, as well as proteins for lipid transport. There was also upregulation during sleep of genes involved in synthesis of proteins, heme, and maintenance of vesicle pools, as well as antioxidant enzymes and genes encoding proteins of energy-regulating pathways. We postulate that during sleep there is a rebuilding of multiple key cellular components in preparation for subsequent wakefulness.