In Silico Structural and Functional Characterization of HtrA Proteins of Leptospira spp.: Possible Implications in Pathogenesis.
In Silico Structural and Functional Characterization of HtrA Proteins of Leptospira spp.: Possible Implications in Pathogenesis.
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钩端螺旋体属HtrA蛋白的电子结构和功能表征：发病机制的可能影响。
DOI：
10.3390/tropicalmed5040179
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发表时间：
2020-11-28
影响因子：
2.9
通讯作者：
Nascimento ALTO
中科院分区：
文献类型：
--
作者：
Daroz BB;Fernandes LGV;Teixeira AF;Nascimento ALTO
钩端螺旋体病是由钩端螺旋体属病原菌引起的人畜共患病。病原菌保守外膜蛋白的鉴定是阐明病原菌致病机制的重要研究目标。表面暴露的蛋白质最有可能参与钩端螺旋体与环境的相互作用。一些螺旋体利用外膜蛋白酶作为穿透宿主组织的一种方式。HtrA是在从原核生物到灵长类动物的各种细胞类型中发现的蛋白质家族。它们是通常由丝氨酸蛋白酶和PDZ结构域组成的一组蛋白酶，并且它们通常被运输到周质。在这里，我们确定了四个基因-注释为HtrA，LIC 11111，LIC 20143，LIC 20144和LIC 11037-和另一个注释为丝氨酸蛋白酶，LIC 11112。据信，后者与LIC 11111形成功能性异源二聚体，因为它们被组织在一个操纵子中。我们的分析表明，这些蛋白质是高度保守的致病菌株。LIC 11112、LIC 20143和LIC 11037具有丝氨酸蛋白酶结构域，并具有保守的催化三联体His-Asp-Ser。需要进行实验研究来阐明这种可能性。
Leptospirosis is a zoonosis caused by the pathogenic bacteria of the genus Leptospira. The identification of conserved outer membrane proteins among pathogenic strains is a major research target in elucidating mechanisms of pathogenicity. Surface-exposed proteins are most probably the ones involved in the interaction of leptospires with the environment. Some spirochetes use outer membrane proteases as a way to penetrate host tissues. HtrA is a family of proteins found in various cell types, from prokaryotes to primates. They are a set of proteases usually composed of a serine protease and PDZ domains, and they are generally transported to the periplasm. Here, we identified four genes—annotated as HtrA, LIC11111, LIC20143, LIC20144 and LIC11037—and another one annotated as a serine protease, LIC11112. It is believed that the last forms a functional heterodimer with LIC11111, since they are organized in one operon. Our analyses showed that these proteins are highly conserved among pathogenic strains. LIC11112, LIC20143, and LIC11037 have the serine protease domain with the conserved catalytic triad His-Asp-Ser. This is the first bioinformatics analysis of HtrA proteins from Leptospira that suggests their proteolytic activity potential. Experimental studies are warranted to elucidate this possibility.
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影响因子：
6.4
作者：
Coleman JL;Toledo A;Benach JL
通讯作者：
Benach JL
弯曲杆菌的弯曲杆菌在极化上皮细胞上的快速细胞跨移民不影响TER：蛋白水解活性HTRA裂解E-钙粘蛋白的作用，而不是纤连蛋白。
影响因子：
4.2
作者：
Boehm M;Hoy B;Rohde M;Tegtmeyer N;Bæk KT;Oyarzabal OA;Brøndsted L;Wessler S;Backert S
通讯作者：
Backert S
影响因子：
6.7
作者：
Caimano MJ;Sivasankaran SK;Allard A;Hurley D;Hokamp K;Grassmann AA;Hinton JC;Nally JE
通讯作者：
Nally JE
影响因子：
3.8
作者：
CHATFIELD, SN;STRAHAN, K;DOUGAN, G
通讯作者：
DOUGAN, G
影响因子：
3.1
作者：
Cortés, G;de Astorza, B;Albertí, S
通讯作者：
Albertí, S