QUANTITATIVE DIFFERENTIATION OF ALPHA-ADRENERGIC AND BETA-ADRENERGIC RESPIRATORY RESPONSES IN ISOLATED HAMSTER BROWN FAT-CELLS - EVIDENCE FOR THE PRESENCE OF AN ALPHA1-ADRENERGIC COMPONENT
QUANTITATIVE DIFFERENTIATION OF ALPHA-ADRENERGIC AND BETA-ADRENERGIC RESPIRATORY RESPONSES IN ISOLATED HAMSTER BROWN FAT-CELLS - EVIDENCE FOR THE PRESENCE OF AN ALPHA1-ADRENERGIC COMPONENT
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DOI：
10.1016/0014-2999(83)90136-x
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发表时间：
1983-01-01
影响因子：
5
通讯作者：
CANNON, B
中科院分区：
文献类型：
--
作者：
MOHELL, N;NEDERGAARD, J;CANNON, B
研究棕色脂肪细胞的呼吸（产热）反应，以区分α-和β-肾上腺素能成分细胞的相对敏感性通常遵循异丙肾上腺素<去甲肾上腺素=肾上腺素. mchlt的EC 50 [中值有效浓度]的模式。对所有这些激动剂的反应对普萘洛尔比对酚妥拉明敏感得多。基于这些标准，反应主要是β 1。剂量-反应曲线和拮抗剂抑制曲线的双相性质另外表明存在α-受体拮抗剂。成分抑制研究证明了IC 50 [中值抑制浓度]系列：哌唑嗪<酚妥拉明<育亨宾，表明α-吲哚美辛组分是α 1-亚型。选择性α-和β-刺激是累加。分离的仓鼠棕色脂肪细胞的最大耗氧量由80%的β 1肾上腺素能成分和20%的α 1肾上腺素能成分组成。不同的机制（β 1通过cAMP和α 1可能通过Ca 2+）和可能不同的目的（例如分别为短期和长期调节）可以解释在1种细胞类型中2种刺激性肾上腺素能应答的共存。
The respiratory (thermogenic) response of brown fat cells was investigated for differentiation between .alpha.- and .beta.-adrenergic components. The relative sensitivity of the cells generally followed the pattern of the EC50 [median effective concentration] for isoprenaline < norepinephrine = epinephrine .mchlt. phenylephrine and the response to all these agonists was much more sensitive to propranolol than to phentolamine. Based on these criteria the response was primarily .beta.1. The biphasic nature of the dose-response curves and the antagonist inhibition curves indicated additionally the presence of an .alpha.-component. Inhibition studies demonstrated the IC50 [median inhibitory concentration] series: prazosin < phentolamine < yohimbine, indicating that the .alpha.-component is of the .alpha.1-subtype. The effects of selective .alpha.- and .beta.-stimulation were additive. The maximal oxygen consumption of isolated hamster brown fat cells was composed of an 80% .beta.1 adrenergic component and a 20% .alpha.1 adrenergic component. Different mechanisms (.beta.1 through cAMP and .alpha.1 possibly through Ca2+) and perhaps different purposes (e.g. short-term and long-term regulation, respectively) may explain the coexistence of 2 stimulatory adrenergic responses in 1 cell type.