Dynamic imaging of T cell-dendritic cell interactions in lymph nodes
Dynamic imaging of T cell-dendritic cell interactions in lymph nodes
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DOI：
10.1126/science.1071065
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发表时间：
2002-06-07
期刊：
影响因子：
56.9
通讯作者：
Germain, RN
中科院分区：
文献类型：
--
作者：
Stoll, S;Delon, J;Germain, RN
T细胞免疫反应始于有组织的淋巴组织。到目前为止，这些反应背后的细胞相互作用的速度、拓扑结构和结果都是从切片组织的静态成像或培养细胞的研究中推断出来的。在这里，我们报告了抗原特异性T细胞与完整外植淋巴结内树突状细胞相互作用的动态可视化。我们观察到免疫突触的形成和这两种线圈类型之间的长时间相互作用，随后是T细胞的激活、解离和快速迁移，远离抗原刺激。这种高分辨率的时空分析提供了对淋巴结构内早期免疫反应至关重要的细胞相互作用的本质的见解。
T cell immune responses begin within organized lymphoid tissues. The pace, topology, and outcomes of the cellular interactions that underlie these responses have, so far, been inferred from static imaging of sectioned tissue or from studies of cultured cells. Here we report dynamic visualization of antigen-specific T cells interacting with dendritic cells within intact explanted lymph nodes. We observed immunological synapse formation and prolonged interactions between these two coil types, followed by the activation, dissociation, and rapid migration of T cells away from the antigenic stimulus. This high-resolution spatiotemporal analysis provides insight into the nature of cell interactions critical to early immune responses within lymphoid structures.