Gene expression analysis of Alcaligenes faecalis during induction of heterotrophic nitrification.
Gene expression analysis of Alcaligenes faecalis during induction of heterotrophic nitrification.
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DOI：
10.1038/s41598-021-02579-3
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发表时间：
2021-11-29
影响因子：
4.6
通讯作者：
Fujiwara T
中科院分区：
文献类型：
--
作者：
Tsujino S;Dohra H;Fujiwara T
粪产碱菌是一种异养硝化细菌，能氧化氨并产生亚硝酸盐和硝酸盐。当A.在以丙酮酸和氨为唯一碳源和氮源的培养基中培养粪产气荚膜梭菌（faecalis），在对数生长期，C/N比小于10的培养基中积累了大量的亚硝酸盐，而C/N比大于15的培养基中未观察到亚硝酸盐的积累。比较转录组分析使用硝化和非硝化细胞的A。在C/N分别为5和20的培养基中培养的faecalis，以评估异养硝化诱导过程中基因表达的波动。在这两种条件下，在指数生长期的细胞中，参与初级代谢的基因的表达水平没有显著变化。我们观察到的四个基因簇的表达水平显着增加：豆荚簇含有的基因编码的磷酸二氢钾肟双加氧酶（POD），podh簇含有的基因编码的POD同系物（PODh），suf簇参与铁硫簇生物合成，和dnf簇参与一种新的羟胺氧化途径在硝化细胞。本研究结果为了解异养硝化的生化机理提供了有价值的信息。
Alcaligenes faecalis is a heterotrophic nitrifying bacterium that oxidizes ammonia and generates nitrite and nitrate. When A. faecalis was cultivated in a medium containing pyruvate and ammonia as the sole carbon and nitrogen sources, respectively, high concentrations of nitrite accumulated in the medium whose carbon/nitrogen (C/N) ratio was lower than 10 during the exponential growth phase, while the accumulation was not observed in the medium whose C/N ratio was higher than 15. Comparative transcriptome analysis was performed using nitrifying and non-nitrifying cells of A. faecalis cultivated in media whose C/N ratios were 5 and 20, respectively, to evaluate the fluctuations of gene expression during induction of heterotrophic nitrification. Expression levels of genes involved in primary metabolism did not change significantly in the cells at the exponential growth phase under both conditions. We observed a significant increase in the expression levels of four gene clusters: pod cluster containing the gene encoding pyruvic oxime dioxygenase (POD), podh cluster containing the gene encoding a POD homolog (PODh), suf cluster involved in an iron-sulfur cluster biogenesis, and dnf cluster involved in a novel hydroxylamine oxidation pathway in the nitrifying cells. Our results provide valuable insight into the biochemical mechanism of heterotrophic nitrification.
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