A soxRS-constitutive mutation contributing to antibiotic resistance in a clinical isolate of Salmonella enterica (serovar Typhimurium)
A soxRS-constitutive mutation contributing to antibiotic resistance in a clinical isolate of Salmonella enterica (serovar Typhimurium)
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DOI：
10.1128/aac.45.1.38-43.2001
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发表时间：
2001-01-01
影响因子：
4.9
通讯作者：
Demple, B
中科院分区：
文献类型：
--
作者：
Koutsolioutsou, A;Martins, EA;Demple, B
soxRS调节子被氧化还原循环药物如百草枯和一氧化氮激活。该系统的>15个基因提供对氧化剂和多种抗生素的抗性。在大肠杆菌中观察到临床喹诺酮耐药与soxRS调节子表达升高之间的关联，但尚未对其他肠道致病菌探索这种关联。在这里，我们描述了一个soxRS组成型突变的临床菌株沙门氏菌肠（血清型鼠伤寒），出现了耐药性的发展，喹诺酮类药物治疗期间。该菌株中升高的喹诺酮耐药性源自soxR基因中的点突变，并且可以被多拷贝野生型soxRS反式抑制。多重抗生素耐药性也转移到实验室菌株的S。通过引入来自临床菌株的克隆的突变soxR基因，结果表明，soxRS的组成型表达可能有助于临床相关的S.肠
The soxRS regulon is activated by redox-cycling drugs such as paraquat and by nitric oxide. The >15 genes of this system provide resistance to both oxidants and multiple antibiotics. An association between clinical quinolone resistance and elevated expression of the soxRS regulon has been observed in Escherichia coli, but this association has not been explored for other enteropathogenic bacteria. Here we describe a soxRS-constitutive mutation in a clinical strain of Salmonella enterica (serovar Typhimurium) that arose with the development of resistance to quinolones during treatment. The elevated quinolone resistance in this strain derived from a point mutation in the soxR gene and could be suppressed in trans by multicopy wild-type soxRS.. Multiple-antibiotic resistance was also transferred to a laboratory strain of S. enterica by introducing the cloned mutant soxR gene from the clinical strain. The results show that constitutive expression of soxRS can contribute to antibiotic resistance in clinically relevant S. enterica.