Specific antigen/antibody interactions measured by force microscopy
Specific antigen/antibody interactions measured by force microscopy
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DOI：
10.1016/s0006-3495(96)79814-4
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发表时间：
1996-05-01
影响因子：
3.4
通讯作者：
Guntherodt, HJ
中科院分区：
文献类型：
--
作者：
Dammer, U;Hegner, M;Guntherodt, HJ
在各种缓冲液条件下，利用原子力显微镜（AFM）直接测量了生物素化牛血清白蛋白与多克隆生物素导向IgG抗体之间的分子识别。研究发现，当在力模式下用原子力显微镜探测时，即使是像IgG抗体这样高度结构化的分子也能保持其对抗原的特异性亲和力。我们能够测量单个抗体 - 抗原复合物之间的断裂力。讨论了这种用于测量单个抗原/抗体相互作用的新方法的潜力和局限性以及一些可能的应用。
Molecular recognition between biotinylated bovine serum albumin and polyclonal, biotin-directed IgG antibodies has been measured directly under various buffer conditions using an atomic force microscope (AFM). It was found that even highly structured molecules such as IgG antibodies preserve their specific affinity to their antigens when probed with an AFM in the force mode. We could measure the rupture force between individual antibody-antigen complexes. The potential and limitations of this new approach for the measurement of individual antigen/antibody interactions and some possible applications are discussed.