Developmental Progression to Infectivity in Trypanosoma brucei Triggered by an RNA-Binding Protein
Developmental Progression to Infectivity in Trypanosoma brucei Triggered by an RNA-Binding Protein
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DOI：
10.1126/science.1229641
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发表时间：
2012-12-07
期刊：
影响因子：
56.9
通讯作者：
Tschudi, Christian
中科院分区：
文献类型：
--
作者：
Kolev, Nikolay G.;Ramey-Butler, Kiantra;Tschudi, Christian
解开导致非洲锥虫病的原生动物寄生虫布氏锥虫与采采蝇（舌蝇属）媒介之间复杂的相互作用仍然是一个挑战。寄生于采采蝇唾液腺的后循环锥虫会传播疾病，它们是通过一个复杂的分化过程和未知的程序产生的。通过在培养的非感染性锥虫中过表达一种单一的RNA结合蛋白TbRBP6，我们重现了在采采蝇中观察到的发育阶段，包括产生表达变异表面糖蛋白的感染性后循环形式。因此，现在可以在实验室中研究导致昆虫媒介获得感染性的事件，为新的干预策略提供了契机。
Unraveling the intricate interactions between Trypanosoma brucei, the protozoan parasite causing African trypanosomiasis, and the tsetse (Glossina) vector remains a challenge. Metacyclic trypanosomes, which inhabit the tsetse salivary glands, transmit the disease and are produced through a complex differentiation and unknown program. By overexpressing a single RNA-binding protein, TbRBP6, in cultured noninfectious trypanosomes, we recapitulated the developmental stages that have been observed in tsetse, including the generation of infective metacyclic forms expressing the variant surface glycoprotein. Thus, events leading to acquisition of infectivity in the insect vector are now accessible to laboratory investigation, providing an opening for new intervention strategies.