A soft, stretchable and conductive biointerface for cell mechanobiology
A soft, stretchable and conductive biointerface for cell mechanobiology
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DOI：
10.1007/s10544-015-9950-0
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发表时间：
2015-04-01
影响因子：
2.8
通讯作者：
Beccai, Lucia
中科院分区：
文献类型：
--
作者：
Bernardeschi, Irene;Greco, Francesco;Beccai, Lucia
在机械生物学中，研究细胞对机械刺激的反应是基本的，所涉及的过程（即机械转导）需要通过在动态和非侵入性自然条件下与细胞（机械和电）连接来研究。在这项工作中，我们提出了一种新颖的柔软，可拉伸和导电的生物界面，可以同时进行细胞机械刺激和动态阻抗记录。通过利用预拉伸130 μ m厚的聚二甲基硅氧烷（PDMS）底物上90 nm厚的聚（3,4-乙烯二氧噻吩）：聚（苯乙烯磺酸酯）（PEDOT:PSS）导电层表面形成的皱纹，获得了生物界面拉伸性和导电性，以及进行细胞培养研究所需的生物相容性和透明度。观察SH-SY5Y人神经母细胞瘤细胞的粘附和增殖情况，观察波纹表面细胞分化情况。我们展示了当施加高达10%的单轴应变和进行细胞培养时，生物界面如何保持导电。最后，由于细胞的机械刺激，相对于对照组，测量到相对阻抗变化信号减少了约30%。
In mechanobiology the study of cell response to mechanical stimuli is fundamental, and the involved processes (i.e., mechanotransduction) need to be investigated by interfacing (mechanically and electrically) with the cells in dynamic and non-invasive natural-like conditions. In this work, we present a novel soft, stretchable and conductive biointerface that allows both cell mechanical stimulation and dynamic impedance recording. The biointerface stretchability and conductivity, jointly to the biocompatibility and transparency needed to perform cell culture studies, were obtained by exploiting the formation of wrinkles on the surface of a 90 nm thick conductive layer of poly(3,4-ethylenedioxythiophene): poly(styrene sulfonate) (PEDOT:PSS) on a pre-stretched 130 mu m thick poly(dimethylsiloxane) (PDMS) substrate. Cell adhesion and proliferation of SH-SY5Y human neuroblastoma cells were evaluated, and cell differentiation on the corrugated surface was assessed. We demonstrate how the biointerface remains conductive when applying uniaxial strain up to 10 %, and when cell culturing is performed. Finally, a reduction of about 30 % of the relative impedance variation signal was measured, with respect to the control, as a result of the mechanical stimulation of cells.