Breastfeeding regulates development of immune system through TGF-β in mice pups
Breastfeeding regulates development of immune system through TGF-β in mice pups
复制标题
母乳喂养通过 TGF-β 调节幼鼠免疫系统的发育
DOI：
10.1111/ped.13507
复制
发表时间：
2018
期刊：
影响因子：
1.4
通讯作者：
Miyake Sachiko
中科院分区：
文献类型：
--
作者：
Sakaguchi Keita;Koyanagi Akemi;Kamachi Fumitaka;Harauma Akiko;Chiba Asako;Hisata Ken;Moriguchi Toru;Shimizu Toshiaki;Miyake Sachiko
研究背景母乳中含有重要的营养成分和免疫调节因子，是新生儿必需的。最近，流行病学研究表明，母乳喂养可以预防广泛的传染病，降低婴儿过敏性疾病的发病率。MethodsTo检查母乳对婴儿期免疫发育的影响，我们建立了一个人工饲养系统的手喂养小鼠和比较喂养的小鼠幼崽无论是母乳或牛奶替代品。所有小鼠在14天的年龄和免疫细胞在胸腺，脾脏和小肠进行了检查流式cytometer.ResultsThe胸腺细胞的数量较高，而外周淋巴组织的总免疫细胞数较低，母乳喂养的小鼠相比，代乳品喂养的小鼠。在外周淋巴组织中，母乳喂养小鼠的B细胞比例较高，CD 8 +T细胞、巨噬细胞、树突状细胞和粒细胞比例显著较低。在用抗转化生长因子β（anti-TGF-β）单克隆抗体处理的母鼠饲养的幼仔中，也观察到母乳替代品喂养的小鼠胸腺和外周淋巴组织免疫细胞的相同变化。因此，母乳中的TGF-β可能是婴儿先天免疫系统介导的疾病的新治疗靶点。
BackgroundBreast milk contains important nutrients and immunoregulatory factors that are essential for newborn infants. Recently, epidemiological studies suggested that breast‐feeding prevents a wide range of infectious diseases and lowers the incidence of infant allergic diseases.MethodsTo examine the effects of breast milk on immunological development in infancy, we established an artificial rearing system for hand‐feeding mice and compared mouse pups fed with either breast milk or milk substitute. All mice were killed at 14 days of age and immune cells in the thymus, spleen, and small intestine were examined on flow cytometry.ResultsThe number of thymocytes was higher whereas that of total immune cells of peripheral lymphoid tissues was lower in mice fed breast milk compared with milk substitute‐fed mice. In peripheral lymphoid tissues, the proportion of B cells was higher and that of CD8+T cells, macrophages, dendritic cells, and granulocytes was significantly lower in breast milk‐fed mice. The same alteration in immune cells of the thymus and peripheral lymphoid tissues in milk substitute‐fed mice was also observed in pups reared by mother mice treated with anti‐transforming growth factor‐β (anti‐TGF‐β) monoclonal antibody.ConclusionsBreast milk regulates the differentiation and expansion of innate and adaptive immune cells partly due to TGF‐β. Hence, TGF‐β in breast milk may be a new therapeutic target for innate immune system‐mediated diseases of infancy.