Polymerase chain reaction assay identifies North American members of the Culex pipiens complex based on nucleotide sequence differences in the acetylcholinesterase gene Ace.2.
Polymerase chain reaction assay identifies North American members of the Culex pipiens complex based on nucleotide sequence differences in the acetylcholinesterase gene Ace.2.
复制标题
聚合酶链式反应分析根据乙酰胆碱酯酶基因 Ace.2 的核苷酸序列差异来鉴定淡色库蚊复合体的北美成员。
DOI：
--
复制
发表时间：
2003
影响因子：
1
通讯作者：
H. M. Savage
中科院分区：
文献类型：
--
作者：
Stephen E. Aspen;H. M. Savage
利用乙酰胆碱酯酶基因 Ace.2 中的核苷酸序列差异开发了一种方法来区分北美淡色库蚊复合体。基于 Ace.2 内含子 2 内序列差异的分类单元特异性聚合酶链反应引物可区分兄弟物种 Cx。 pipiens Linneaus 和 Cx。 quinquefasciatus Say 及其 F1 杂交种。该测定可用于确认蚊子池的物种组成，识别在虫媒病毒监测计划和其他蚊子研究中收集的个体样本，并确定杂交区域。
Nucleotide sequence differences in the acetylcholinesterase gene Ace.2 were used to develop an assay to distinguish among North American mosquitoes of the Culex pipiens complex. Taxon-specific polymerase chain reaction primers based on sequence differences within intron 2 of Ace.2 distinguish among the sibling species Cx. pipiens Linneaus and Cx. quinquefasciatus Say and their F1 hybrids. This assay may be used to confirm the species composition of mosquito pools, identify individual specimens collected in arbovirus surveillance programs and other mosquito studies, and define zones of hybridization.