Intermittent posterior displacement of the rat mandible in the growth period affects the condylar cancellous bone
Intermittent posterior displacement of the rat mandible in the growth period affects the condylar cancellous bone
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DOI：
10.2319/122810-749.1
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发表时间：
2011-11-01
影响因子：
3.4
通讯作者：
Ono, Takashi
中科院分区：
文献类型：
--
作者：
Kuroda, Yukiko;Yonemitsu, Ikuo;Ono, Takashi
目的：为了研究是否间歇性后髁移位引起的变化，在下颌骨髁状突在growth.Materials和方法：16 5周龄雄性Wistar大鼠分为实验组和对照组。在实验组中，将一个矫治器连接到上颌切牙上，以在咬合条件下诱导髁状突后移。未处理的大鼠作为对照组。在第14天处死动物，取出髁突，通过显微计算机断层扫描（micro-CT）分析三维松质骨结构。连续矢状面石蜡切片的髁状突进行苏木精和伊红染色，以探讨组织形态学的变化和抗酒石酸酸性磷酸酶（TRAP）染色，以确定osteocytes cells.Results：Micro-CT分析显示，在实验组中，骨体积分数和各向异性程度显着降低，与对照组相比，在前区的髁状突。此外，TRAP阳性细胞的数量显着大于在同一地区的实验组比对照组。结论：间歇性后移位的下颌骨可以引起特定区域的轮廓和微结构的髁状突松质骨的变化。（角度正交。2011;81：975-982）。
Objective: To examine whether intermittent posterior condylar displacement causes changes in cancellous bone in the mandibular condyle during the growth period.Materials and Methods: Sixteen 5-week-old male Wistar rats were divided into experimental and control groups. In the experimental group, an appliance was attached to the maxillary incisors to induce posterior displacement of the condyles in the occluded condition. Untreated rats served as the control group. Animals were sacrificed at 14 days, and the condyles were removed to analyze the three-dimensional cancellous bone structure by microcomputed tomography (micro-CT). Serial sagittal paraffin sections of the condyles were used for hematoxylin and eosin staining to investigate histomorphological changes and for tartrate-resistant acid phosphatase (TRAP) staining to identify osteoclastic cells.Results: Micro-CT analysis showed that in the experimental group, the bone volume fraction and the degree of anisotropy were significantly decreased compared with those in the control group in the anterior region of the condyle. Moreover, the number of TRAP-positive cells was significantly greater in the same region in the experimental group than in the control group.Conclusion: Intermittent posterior displacement of the mandible can cause region-specific changes in the profile and microarchitecture of the condylar cancellous bone. (Angle Orthod. 2011;81:975-982.)