Digital photo-control of the network of live excitable cells
Digital photo-control of the network of live excitable cells
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活体可兴奋细胞网络的数字光控制
DOI：
10.1134/s0021364011180056
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发表时间：
2011
期刊：
影响因子：
--
通讯作者：
K.Agladze
中科院分区：
文献类型：
--
作者：
I.S.Erofeev;N.Magome;K.Agladze
组织工程技术的最新发展允许创建和维持几乎无限期的具有所需结构的可兴奋细胞网络。我们将活的可兴奋心脏细胞网络与普通计算机耦合，方法是使它们对光敏感，在细胞层上投射光图案，并借助荧光探针（光学映射）监测激发。作为敏化物质，我们使用偶氮苯三甲基溴化铵（AzoTAB）。这种物质经历顺-反光异构化，AzoTAB的反式异构体抑制心肌细胞的兴奋，而顺式异构体不抑制。因此，AzoTAB介导的致敏允许可逆和动态地控制通过整个心肌细胞网络的激发波，或者均匀地，或者以优选的空间模式。从技术上讲，它是通过将一个普通的数字投影仪与一个宏观显微镜相结合，并使用计算机图形软件来创建传导路径的投影图案来实现的。这种方法允许心脏组织的真实的实时交互式光控制。
Recent development of tissue engineering techniques allows creating and maintaining almost indefinitely networks of excitable cells with desired architecture. We coupled the network of live excitable cardiac cells with a common computer by sensitizing them to light, projecting a light pattern on the layer of cells, and monitoring excitation with the aid of fluorescent probes (optical mapping). As a sensitizing substance we used azobenzene trimethylammonium bromide (AzoTAB). This substance undergoescis-trans-photoisomerization andtrans-isomer of AzoTAB inhibits excitation in the cardiac cells, whilecis-isomer does not. AzoTAB-mediated sensitization allows, thus, reversible and dynamic control of the excitation waves through the entire cardiomyocyte network either uniformly, or in a preferred spatial pattern. Technically, it was achieved by coupling a common digital projector with a macroview microscope and using computer graphic software for creating the projected pattern of conducting pathways. This approach allows real time interactive photocontrol of the heart tissue.