Distinct inflammatory profiles in atopic and nonatopic patients with chronic rhinosinustis accompanied by nasal polyps in Western china.
Distinct inflammatory profiles in atopic and nonatopic patients with chronic rhinosinustis accompanied by nasal polyps in Western china.
复制标题
中国西部特应性和非特应性慢性鼻窦炎伴鼻息肉患者的不同炎症特征
DOI：
10.4168/aair.2015.7.4.346
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发表时间：
2015-07
期刊：
影响因子：
--
通讯作者：
Li H
中科院分区：
文献类型：
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作者：
Ba L;Du J;Liu F;Yang F;Han M;Liu S;Lin P;Li H
目的 全身致敏在慢性鼻窦炎伴鼻息肉 (CRSwNP) 病理生理学中的作用仍然难以捉摸。本研究旨在表征 CRSwNP 特应性和非特应性患者息肉组织中细胞因子的模式。方法 分别收集 70 例 CRSwNP 患者和 26 例对照受试者的特应性和非特应性息肉和正常组织。采用免疫组织化学法检测炎症细胞（嗜酸性粒细胞、中性粒细胞、肥大细胞等）的分布，采用实时定量聚合酶链式反应测定转录因子GATA-3、T-bet、RORc和FOXP3的mRNA水平。采用酶联免疫吸附试验（ELISA）测定组织匀浆中炎症介质（IFN-γ、IL-5、IL-17A等）的水平。此外，使用 ELISA 测量抗 IgE 刺激的息肉组织上清液中炎症介质的水平。结果 特应性CRSwNP患者的特点是嗜酸性粒细胞积聚增加，嗜酸性粒细胞炎症增强（IL-5、ECP和总IgE升高），GATA-3 mRNA水平显着升高（P<0.05），而特应性和非特应性CRSwNP患者均表现出FOXP3 mRNA表达降低（P<0.05）。添加抗 IgE 刺激后，特应性 CRSwNP 患者在刺激的息肉组织上清液中比非特应性 CRSwNP 患者产生更多的 IL-5、IL-2、IL-10、IL-17A 和 PGD2。结论 特应性和非特应性CRSwNP患者可能具有息肉组织的炎症反应模式。
Purpose The role of systemic sensitization in the pathophysiology of chronic rhinosinusitis with nasal polyps (CRSwNP) remains elusive. This study sought to characterize the pattern of cytokines in polyp tissues from atopic and nonatopic patients with CRSwNP. Methods Atopic and nonatopic polyp and normal tissues were collected from 70 CRSwNP patients and 26 control subjects, respectively. The distribution of inflammatory cells (eosinophils, neutrophils, mast cells, etc.) were examined using immunohistochemistry, the mRNA levels of the transcription factors GATA-3, T-bet, RORc, and FOXP3 were determined using quantitative real-time polymerase chain reaction. The levels of inflammatory mediators (IFN-γ, IL-5, IL-17A, etc.) in tissue homogenates were measured using enzyme-linked immunosorbent assay (ELISA). Moreover, the levels of inflammatory mediators in the supernatant of anti-IgE stimulated polyp tissues were measured using ELISA. Results Atopic CRSwNP patients were characterized by increased eosinophil accumulation, enhanced eosinophilic inflammation (elevated IL-5, ECP, and total IgE), and significantly increased GATA-3 mRNA levels (P<0.05), whereas both atopic and non-atopic CRSwNP patients showed decreased FOXP3 mRNA expression (P<0.05). After addition of anti-IgE stimulation, atopic CRSwNP patients produced more IL-5, IL-2, IL-10, IL-17A, and PGD2 in the supernatant of stimulated polyp tissues than nonatopic CRSwNP patients did. Conclusions Atopic and nonatopic CRSwNP patients may possess the patterns of inflammatory response in polyp tissues.