Morphological study of erythrocyte shapes in red pulp of mouse spleens revealed by an in vivo cryotechnique.
Morphological study of erythrocyte shapes in red pulp of mouse spleens revealed by an in vivo cryotechnique.
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通过体内冷冻技术揭示小鼠脾红髓中红细胞形状的形态学研究。
DOI：
10.14670/hh-16.123
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发表时间：
2001
影响因子：
2
通讯作者：
Shinichi Ohno
中科院分区：
文献类型：
--
作者：
Mei Xue;T. Baba;N. Terada;Y. Kato;Y. Fujii;Shinichi Ohno
本研究的目的是使用我们的体内冷冻技术和扫描 (SEM) 或透射 (TEM) 电子显微镜来阐明活体小鼠脾脏脾索中的红细胞形状。一些小鼠的脾脏在麻醉下心脏跳动的情况下通过体内冷冻技术快速冷冻。相比之下，其他脾脏是从腹腔取出后通过体外冷冻方法制备的。它们通常被冷冻替代，并为 SEM 和 TEM 做好准备。一些小鼠脾脏也被常规固定并包埋在Quetol-812中以获得常规形态。活体小鼠脾脏中的红细胞呈现出多种形状，它们之间的空间狭窄，被困在网状纤维组织中。采用体外冷冻法制备的红髓，即使阻断正常血液循环，仍保留类似的各种形状的红细胞。与上述结果相比，传统的化学固定剂浸没固定使一些红细胞变成双凹盘状。它们还在网状纤维组织之间显示出彼此之间的宽阔空间。这种传统的形态学研究很难揭示血液循环正常的功能性脾脏中红细胞的体内形状。相比之下，我们的体内冷冻技术证明了功能性脾脏中红细胞的各种形状。研究表明，尽管脾脏血液循环的微环境发生了变化，但大多数在脾脏中充血的红细胞仍保持其原始形态。
The purpose of this study was to clarify erythrocyte shapes in splenic cords of living mouse spleens, using our in vivo cryotechnique followed by scanning (SEM) or transmission (TEM) electron microscopy. Some spleens of mice were quickly frozen by the in vivo cryotechnique while their hearts were beating under anesthesia. In contrast, other spleens were prepared by an in vitro freezing method after they were taken out from the abdominal cavity. They were routinely freeze-substituted, and prepared for SEM and TEM. A few mouse spleens were also routinely fixed and embedded in Quetol-812 to obtain conventional morphology. Erythrocytes in living mouse spleens showed a variety of shapes with narrow spaces between them, trapped among reticular fiber tissues. Similar various shapes of erythrocytes were kept in the red pulp even after blocking normal blood circulation, as prepared by the in vitro freezing method. In comparison to the above-mentioned findings, some erythrocytes were changed to biconcave discoid shapes by the conventional immersion fixation with chemical fixatives. They also showed wide spaces between each other among reticular fiber tissues. Such conventional morphological studies could hardly reveal the in vivo shapes of erythrocytes in functioning spleens with normal blood circulation. In contrast, the various shapes of erythrocytes in the functioning spleens were demonstrated by our in vivo cryotechnique. It is suggested that most erythrocytes congesting in spleens keep their original configuration in spite of microenviromental alteration in splenic blood circulation.
一种简单的气动装置，用于冷冻在电子显微镜网格上生长的细胞。
DOI：
10.1002/jemt.1060160207
发表时间：
1990
期刊：
Journal of electron microscopy technique
影响因子：
--
作者：
Cole,R;Matuszek,G;See,C;Rieder,CL
通讯作者：
Rieder,CL