Reaction-Based Off On Near-infrared Fluorescent Probe for Imaging Alkaline Phosphatase Activity in Living Cells and Mice
Reaction-Based Off On Near-infrared Fluorescent Probe for Imaging Alkaline Phosphatase Activity in Living Cells and Mice
复制标题
基于反应的关断近红外荧光探针，用于活细胞和小鼠中碱性磷酸酶活性的成像
DOI：
10.1021/acsami.6b14176
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发表时间：
2017-03-01
影响因子：
9.5
通讯作者：
Sun, Hongyan
中科院分区：
文献类型：
--
作者：
Tan, Yi;Zhang, Ling;Sun, Hongyan
碱性磷酸酶是一组在调节多种细胞功能和疾病发病机制中起重要作用的酶。因此，开发荧光探针在体内检测碱性磷酸酶活性是非常可取的研究动态磷酸化在活的生物体。在这里，我们开发了第一个基于近红外（NIR）探针（DHXP）的灵敏检测碱性磷酸酶活性在体外和体内。结果表明，该探针与碱性磷酸酶共孵育后，荧光强度增加了66倍，其检测限为0.07 U/L，低于文献报道的大多数碱性磷酸酶探针的检测限。该探针可用于细胞和小鼠体内碱性磷酸酶活性的检测。此外，我们的探针具有良好的生物相容性和快速的细胞内化能力。鉴于这些突出的特性，我们设想DHXP将为生物医学研究中的碱性磷酸酶活性研究增加有用的工具。
Alkaline phosphatases are a group of enzymes that play important roles in regulating diverse cellular functions and disease pathogenesis. Hence, developing fluorescent probes for in vivo detection of alkaline phosphatase activity is highly desirable for studying the dynamic phosphorylation in living organisms. Here, we developed the very first reaction based near-infrared (NIR) probe (DHXP) for sensitive detection of alkaline phosphatase activity both in vitro and in vivo. Our studies demonstrated that the probe displayed an up to 66-fold fluorescence increment upon incubation with alkaline phosphatases, and the detection limit of our probe was determined to be 0.07 U/L, which is lower than that of most of alkaline phosphatase probes reported in literature. Furthermore, we demonstrated that the probe can be applied to detecting alkaline phosphatase activity in cells and mice. In addition, our probe possesses excellent biocompatibility and rapid cell-internalization ability. In light of these prominent properties, we envision that DHXP will add useful tools for investigating alkaline phosphatase activity in biomedical research.