Bioprinted Chitosan and Hydroxyapatite Micro-Channels Structures Scaffold for Vascularization of Bone Regeneration
Bioprinted Chitosan and Hydroxyapatite Micro-Channels Structures Scaffold for Vascularization of Bone Regeneration
复制标题
生物打印壳聚糖和羟基磷灰石微通道结构用于骨再生血管化的支架
DOI：
10.1166/jbt.2017.1535
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发表时间：
2017
影响因子：
0.1
通讯作者：
Hu Qingxi
中科院分区：
文献类型：
--
作者：
Liu Change;Liu Yuanyuan;Li Shuai;Sun Yuanshao;Li Yu;Jiang Chen;Hu Qingxi
本研究采用同轴挤压法和水浴交联法相结合的方法制备了中空壳聚糖/羟基磷灰石（CS/HA）纤维复合材料，并利用3D生物打印系统制备了3D支架。通过SEM图像可以清楚地看到中空结构。降解实验证明CS/HA支架具有很高的适应人体环境的潜力，压缩试验结果表明，添加适量的羟基磷灰石提高了力学性能。此外，在培养6天后，中空结构中的细胞活力达到88 ± 6.02%，证明了良好的生物相容性。血管化是至关重要的，负责为所有细胞提供充足的氧气和营养。该研究为制备空心壳聚糖/羟基磷灰石（CS/HA）新型材料，通过构建内置微通道的骨组织工程支架实现血管化提供了一种重要方法。
In this work, we presented a new materials composed of hollow chitosan and hydroxyapatite (CS/HA) fibers were bio-fabricated by combining a coaxial extrusion with a water bath crosslinking and a 3D scaffold was fabricated by a 3D bioprinting system. The hollow structure could be clearly seen by SEM images. Degradation experiments proved that CS/HA scaffold had a high potential to adapt to a human environment and compression tests results revealed that the addition of an appropriate quantity of hydroxyapatite increases the mechanical properties. Moreover, cell viability reached 88 ± 6.02% in the hollow constructs after 6 days of culture, proving good biocompatibility. Vascularization is vital and responsible for the sufficient supply in oxygen and nutrients to all cells. This study provides an important method for the production of a new materials composed of hollow chitosan and hydroxyapatite (CS/HA) to achieve vascularization by fabricating scaffold with built-in micro-channels in bone tissue engineering.