RIBOSOMAL-RNA GENE ACTIVITY AND CONTROL OF EXPRESSION MEDIATED BY METHYLATION AND IMPRINTING DURING EMBRYO DEVELOPMENT IN WHEAT X RYE HYBRIDS
RIBOSOMAL-RNA GENE ACTIVITY AND CONTROL OF EXPRESSION MEDIATED BY METHYLATION AND IMPRINTING DURING EMBRYO DEVELOPMENT IN WHEAT X RYE HYBRIDS
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DOI：
10.1007/bf00222984
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发表时间：
1995-08-01
影响因子：
5.4
通讯作者：
VIEGAS, W
中科院分区：
文献类型：
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作者：
NEVES, N;HESLOPHARRISON, JS;VIEGAS, W
起源于亲本的核糖体RNA基因在种间杂交中经常被抑制。我们表明，在萌发期间用胞嘧啶类似物5-氮杂胞苷处理，通过防止黑麦rDNA中位点的甲基化，稳定地重新激活了小麦x黑麦双二倍体小黑麦中受抑制的黑麦起源的rRNA基因的表达。当5-氮胞苷应用于小黑麦和小麦×黑麦F-1杂种的胚胎，在受精后9天或更长时间，黑麦rRNA基因在所产生的幼苗中稳定地重新激活。早期处理对黑麦rRNA基因表达没有影响，表明胚胎发育早期DNA甲基化不足是可逆的。9天后，rRNA基因的甲基化状态在整个发育过程中保持不变。由于表达的变化是在特定限制酶位置的甲基化变化之后发生的，因此这些数据在植物中的基因活性和甲基化之间建立了明显的相关性。
Ribosomal RNA genes originating from one parent are often suppressed in interspecific hybrids. We show that treatments during germination with the cytosine analogue 5-azacytidine stably reactivate the expression of the suppressed rRNA genes of rye origin in the wheat x rye amphiploid, triticale, by preventing methylation of sites in the rye rDNA. When 5-azacytidine is applied to embryos of triticale and wheat x rye F-1 hybrids nine, or more, days after fertilization, rye rRNA gene expression is stably reactivated in the resulting seedling. Earlier treatments have no effect on rye rRNA gene expression, indicating that undermethylation of DNA early in embryo development is reversible. After 9 days, the methylation status of rRNA genes in maintained throughout development. Since the change in expression follows a methylation change at particular restriction-enzyme sites, the data establish a clear correlation between gene activity and methylation in plants.