Aedes aegypti: an emerging model for vector mosquito development.
Aedes aegypti: an emerging model for vector mosquito development.
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DOI：
10.1101/pdb.emo141
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发表时间：
2010-10-01
影响因子：
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通讯作者：
Duman-Scheel M
中科院分区：
文献类型：
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作者：
Clemons A;Haugen M;Flannery E;Tomchaney M;Kast K;Jacowski C;Le C;Mori A;Simanton Holland W;Sarro J;Severson DW;Duman-Scheel M
吸血蚊子，包括登革热和黄热病媒介埃及伊蚊，传播世界上许多最致命的疾病。此类疾病在发展中国家卷土重来，对发达国家的流行病爆发构成明显威胁。最近的蚊子基因组计划激发了人们对通过媒介昆虫的基因操作控制节肢动物传播疾病的潜力的兴趣。特别感兴趣的目标包括调节发育的基因。然而，虽然Ae.埃及伊蚊基因组计划发现了许多已知发育调控基因的同源物，但人们对伊蚊发育的遗传调控知之甚少。埃及伊蚊或其他媒介蚊子。本文概述了 Ae 的背景、饲养和潜在用途。埃及伊蚊作为模式物种。培养、收集和固定发育组织、分析基因和蛋白质表达以及敲除基因的方法可以详细分析发育调节基因的功能以及在白斑病期间对此类基因的选择性抑制。埃及伊蚊的发展。这种方法大部分适用于其他蚊子物种，对于比较发展和媒介研究界都很有用。
Blood-feeding mosquitoes, including the dengue and yellow fever vector Aedes aegypti, transmit many of the world’s deadliest diseases. Such diseases have resurged in developing countries and pose clear threats for epidemic outbreaks in developed countries. Recent mosquito genome projects have stimulated interest in the potential for arthropod-borne disease control by genetic manipulation of vector insects. Targets of particular interest include genes that regulate development. However, although the Ae. aegypti genome project uncovered homologs of many known developmental regulatory genes, little is known of the genetic regulation of development in Ae. aegypti or other vector mosquitoes. This article provides an overview of the background, husbandry, and potential uses of Ae. aegypti as a model species. Methods for culturing, collecting and fixing developing tissues, analyzing gene and protein expression, and knocking down genes are permitting detailed analyses of the functions of developmental regulatory genes and the selective inhibition of such genes during Ae. aegypti development. This methodology, much of which is applicable to other mosquito species, is useful to both the comparative development and vector research communities.