Chloroplast-avoidance response induced by high-fluence blue light in prothallial cells of the fern Adiantum capillus-veneris as analyzed by microbeam irradiation
Chloroplast-avoidance response induced by high-fluence blue light in prothallial cells of the fern Adiantum capillus-veneris as analyzed by microbeam irradiation
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DOI：
10.1104/pp.119.3.917
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发表时间：
1999-03-01
期刊：
影响因子：
7.4
通讯作者：
Wada, M
中科院分区：
文献类型：
--
作者：
Kagawa, T;Wada, M
利用高通量蓝色微束对蕨类植物铁线蕨光培养和暗适应的原叶细胞进行部分细胞照射，诱导叶绿体运动。光照区域内的叶绿体向外移动(高通量响应[HFR])，而外部的叶绿体向辐射区域移动(低通量响应[LFR])，尽管它们在到达边界时停止移动。这些结果表明，HFR和LFR信号都是由同一区域的高通量蓝光产生的，LFR信号可以从束斑远距离传输，而HFR信号不能。HFR信号的寿命是根据高通量蓝色微束的短脉冲诱导的叶绿体运动的痕迹计算出来的，脉冲时间约为6分钟。与LFR(30-40分钟；T.Kagawa，M.Wada[1994]J植物资源107：389-398)相比，这是非常短的。这些数据表明，HFR和LFR的信号转导途径必须是不同的。
Chloroplast movement was induced by partial cell illumination using a high-fluence blue microbeam in light-grown and dark-adapted prothallial cells of the fern Adiantum capillus-veneris. Chloroplasts inside the illuminated area moved out (high-fluence response [HFR]), whereas those outside moved toward the irradiated area (low-fluence response [LFR]), although they stopped moving when they reached the border. These results indicate that both HFR and LFR signals are generated by high-fluence blue light of the same area, and that an LFR signal can be transferred long-distance from the beam spot, although an HFR signal cannot. The lifetime of the HFR signal was calculated from the traces of chloroplast movement induced by a brief pulse from a high-fluence blue microbeam to be about 6 min. This is very short compared with that of the LFR (30-40 min; T. Kagawa, M. Wada [1994] J Plant Res 107: 389-398). These data indicate that the signal transduction pathways of the HFR and the LFR must be distinct.