Isolation of viral haemorrhagic septicaemia virus from mummichog, stickleback, striped bass and brown trout in eastern Canada
Isolation of viral haemorrhagic septicaemia virus from mummichog, stickleback, striped bass and brown trout in eastern Canada
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DOI：
10.1111/j.1365-2761.2007.00802.x
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发表时间：
2007-04-01
影响因子：
2.5
通讯作者：
Olivier, G.
中科院分区：
文献类型：
--
作者：
Gagne, N.;MacKinnon, A-M;Olivier, G.
病毒性出血性败血症病毒（VHSV）是从加拿大新玩法和新斯科舍省的木乃伊猪、异基底、棘鱼、棘腹鱼、褐鳟鱼、萨尔莫和条纹鲈鱼（Morone saxatilis）种群中发生的死亡病例中分离出来的。分离的病毒株对乳头状上皮瘤细胞系产生细胞病变效应。血清中和表明病毒为VHSV，测序鉴定弹状病毒分离株为北美VHSV株。系统发育分析表明，分离物是密切相关的，并形成一个可区分的亚组的北美型VHSV。据我们所知，这是第一次报告VHSV在mummichog和条纹鲈鱼。
Viral haemorrhagic septicaemia virus (VHSV) was isolated from mortalities occurring in populations of mummichog, Fundulus heteroclitus, stickleback, Gasterosteus aculeatus aculeatus, brown trout, Salmo trutta, and striped bass, Morone saxatilis, in New Brunswick and Nova Scotia, Canada. The isolated viral strains produced a cytopathic effect on the epithelioma papillosum cyprini cell line. Serum neutralization indicated the virus was VHSV and sequencing identified the rhabdovirus isolates as the North American strain of VHSV. Phylogenetic analysis indicated that the isolates are closely related and form a distinguishable subgroup of North American type VHSV. To our knowledge, this is the first report of VHSV in mummichog and striped bass.