REGULATION OF GLYCOGEN-METABOLISM IN PRIMARY AND TRANSFORMED ASTROCYTES INVITRO
REGULATION OF GLYCOGEN-METABOLISM IN PRIMARY AND TRANSFORMED ASTROCYTES INVITRO
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DOI：
10.1111/j.1471-4159.1983.tb12662.x
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发表时间：
1983-01-01
影响因子：
4.7
通讯作者：
PASSONNEAU, JV
中科院分区：
文献类型：
--
作者：
CUMMINS, CJ;LUST, WD;PASSONNEAU, JV
在新生大鼠脑星形胶质细胞的原代培养物和疱疹病毒转化培养物中研究了糖原代谢。在原代和转化的星形胶质细胞培养物中，消耗的葡萄糖的一小部分被保存在糖原中。添加含有 5.5 mM 葡萄糖的培养基后，糖原在 2.5 小时内增加到最大水平，大约是培养基葡萄糖消耗一半的时间，然后在原代和转化的星形胶质细胞培养物中迅速下降。糖原的最大水平显然与疱疹病毒转化培养物的细胞密度相关，但原代培养物并未表现出这种行为。在任何给定的细胞密度下，糖原的最大水平取决于细胞外葡萄糖的浓度。施用葡萄糖引起糖原合成酶a的短暂激活和糖原磷酸化酶a的快速失活。
Glycogen metabolism was studied in primary and Herpesvirus-transformed cultures of neonatal rat brain astrocytes. A small fraction of the glucose consumed was conserved in glycogen in both the primary and the transformed astrocytic cell cultures. After addition of culture medium containing 5.5 mM glucose, glycogen increased to maximal levels within 2.5 h, the approximate time at which half of the medium glucose was consumed, and rapidly declined thereafter in both the primary and transformed astrocytic cultures. Maximum levels of glycogen were apparently related to the cell density of the Herpesvirus-transformed cultures, but primary cultures did not show this behavior. At any given cell density, maximal levels of glycogen were dependent on the concentration of extracellular glucose. Administration of glucose caused a transient activation of glycogen synthase a and a rapid inactivation of glycogen phosphorylase a.