Cholesterol diet enhances daily rhythm of Pai-1 mRNA in the mouse liver
Cholesterol diet enhances daily rhythm of Pai-1 mRNA in the mouse liver
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DOI：
10.1152/ajpendo.00095.2004
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发表时间：
2004-10-01
影响因子：
5.1
通讯作者：
Shibata, S
中科院分区：
文献类型：
--
作者：
Kudo, T;Nakayama, E;Shibata, S
心肌梗塞常发生在早晨，这一现象部分是由于纤溶活性下调所致。纤溶酶原激活物抑制剂-1（派-1）是纤溶活性背后的关键因子，其基因表达受小鼠心脏和肝脏中的生物钟基因控制。由于派-1活性增强，高胆固醇血症与纤维蛋白溶解受损相关，这也与动脉粥样硬化有关。本研究的目的是破译是否派-1基因仍然表达的高胆固醇血症的日常。高胆固醇血症（1%胆固醇饮食）对肝脏时钟基因（Per 2和Bmal 1）和时钟控制基因（Dbp和E4 bp 4）的日表达量无显著影响（P>0.05）;但派-1基因和派-1启动子调控因子基因如Nr 4 a1的日表达量显著上调（P
Myocardial infarction frequently occurs in the morning, a phenomenon in part resulting from the downregulation of fibrinolytic activity. Plasminogen activator inhibitor-1 (PAI-1) is a key factor behind fibrinolytic activity, and its gene expression is controlled under the circadian clock gene in the mouse heart and liver. Hypercholesterolemia has been associated with impaired fibrinolysis due to enhanced PAI-1 activity, which has also been implicated in atherosclerosis. The aim of this study was to decipher whether the Pai-1 gene is still expressed daily with hypercholesterolemia. Hypercholesterolemia (1% cholesterol diet) did not significantly affect the daily expression of clock genes (Per2 and Bmal1) and clock-controlled genes (Dbp and E4bp4) in the liver (P>0.05); however, daily expression of the Pai-1 gene and Pai-1 promoter regulating factor genes such as Nr4a1 was significantly upregulated (P