Cysteine residues in the fourth zinc finger are important for activation of the nitric oxide‐inducible transcription factor Fzf1 in the yeast Saccharomyces cerevisiae
Cysteine residues in the fourth zinc finger are important for activation of the nitric oxide‐inducible transcription factor Fzf1 in the yeast Saccharomyces cerevisiae
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DOI：
10.1111/gtc.12885
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发表时间：
2021-07
期刊：
影响因子：
2.1
通讯作者：
Ryo Nasuno;Natsuko Yoshioka;Y. Yoshikawa;H. Takagi
中科院分区：
文献类型：
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作者：
Ryo Nasuno;Natsuko Yoshioka;Y. Yoshikawa;H. Takagi
一氧化氮（NO）是一种普遍存在于各种生物体中的信号分子。在酿酒酵母中，NO在细胞保护和细胞死亡中起作用，这取决于其浓度。因此，严格调节NO浓度对于酵母细胞是重要的。转录因子Fzf 1含有5个锌指，据报道通过调节编码NO双加氧酶的YHB 1基因的表达对NO稳态很重要。然而，NO激活Fzf 1的机制仍不清楚。在这项研究中，我们通过RT-qPCR和Western blotting表明NO在蛋白水平特异性激活Fzf 1。我们进一步的转录分析表明，第四锌指（ZF 4）中的半胱氨酸残基是Fzf 1的NO响应性激活所必需的。此外，本研究结果表明，ZF 4是重要的蛋白质稳定性的Fzf 1。根据这些结果，我们提出了Fzf 1激活的可能机制。
Nitric oxide (NO) is a ubiquitous signaling molecule in various organisms. In the yeast Saccharomyces cerevisiae, NO functions in both cell protection and cell death, depending on its concentration. Thus, it is important for yeast cells to strictly regulate NO concentration. The transcription factor Fzf1, containing five zinc fingers, is reportedly important for NO homeostasis by regulating the expression of the YHB1 gene, which encodes NO dioxygenase. However, the mechanism by which NO activates Fzf1 is still unclear. In this study, we showed that NO activated Fzf1 specifically at the protein level by RT‐qPCR and Western blotting. Our further transcriptional analyses indicated that cysteine residues in the fourth zinc finger (ZF4) are required for the NO‐responsive activation of Fzf1. Additionally, the present results suggest that ZF4 is important for the protein stability of Fzf1. From these results, we proposed possible mechanisms underlying Fzf1 activation.