IL-13 selectively induces vascular cell adhesion molecule-1 expression in human endothelial cells.
IL-13 selectively induces vascular cell adhesion molecule-1 expression in human endothelial cells.
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DOI：
10.4049/jimmunol.154.2.799
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发表时间：
1995-01
影响因子：
4.4
通讯作者：
B. Bochner;David Klunk;S. A. Sterbinsky;R. Coffman;R. Schleimer
中科院分区：
文献类型：
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作者：
B. Bochner;David Klunk;S. A. Sterbinsky;R. Coffman;R. Schleimer
先前对人脐静脉内皮细胞（HUVEC）的研究表明，细胞因子IL-4诱导人嗜酸性粒细胞粘附，但不能诱导中性粒细胞粘附，因为它能够选择性地诱导血管细胞粘附分子-1 （VCAM-1）的表面表达。由于细胞因子IL-13与IL-4具有许多相同的生物学特性，我们研究了IL-13对HUVEC粘附分子表达和功能的影响。间接免疫荧光和流式细胞术检测，IL-13 （0.1 ~ 15 U/ml）与HUVEC孵育4 ~ 48 h可诱导VCAM-1的表面表达，对e-选择素和细胞间粘附分子-1的表达无显著影响。il -13诱导VCAM-1表达的动力学和最大表达量与IL-4相似。用最佳浓度IL-13 （15 U/ml, 24 h）处理HUVEC可诱导嗜酸性粒细胞粘附，但对中性粒细胞不具有粘附性，并且粘附被识别VCAM-1或α 4整合素（CD49d）的单克隆抗体完全抑制。这些结果表明，IL-13和IL-4一样，选择性地刺激HUVEC表达功能性细胞表面VCAM-1，并提示IL-13可能在体内过敏和其他炎症反应中促进VCAM-1/ α 4整合素依赖的嗜酸性粒细胞积累。
Previous studies with human umbilical vein endothelial cells (HUVEC) have shown that the cytokine IL-4 induces adherence of human eosinophils, but not neutrophils, because of its ability to selectively induce surface expression of vascular cell adhesion molecule-1 (VCAM-1). Because the cytokine IL-13 shares a number of biologic properties with IL-4, we examined the effect of IL-13 on the expression and function of adhesion molecules on HUVEC. Incubation of HUVEC for 4 to 48 h with IL-13 (0.1 to 15 U/ml) induced surface expression of VCAM-1, as detected by indirect immunofluorescence and flow cytometry, without significantly affecting expression of E-selectin or intercellular adhesion molecule-1. The kinetics and maximal IL-13-induced expression of VCAM-1 were similar to those seen with IL-4. Treatment of HUVEC with an optimal concentration of IL-13 (15 U/ml for 24 h) induced adhesiveness for eosinophils, but not for neutrophils, and adhesion was completely inhibited by mAb recognizing VCAM-1 or alpha 4 integrin (CD49d). These results demonstrate that IL-13, like IL-4, selectively stimulates HUVEC to express functional cell surface VCAM-1 and suggest a possible role for IL-13 in promoting VCAM-1/alpha 4 integrin-dependent accumulation of eosinophils during allergic and other inflammatory reactions in vivo.