SPECIFIC RETROGRADE TRANSPORT OF NERVE GROWTH-FACTOR (NGF) FROM NEOCORTEX TO NUCLEUS BASALIS IN THE RAT
SPECIFIC RETROGRADE TRANSPORT OF NERVE GROWTH-FACTOR (NGF) FROM NEOCORTEX TO NUCLEUS BASALIS IN THE RAT
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DOI：
10.1016/0006-8993(84)91338-6
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发表时间：
1984-01-01
期刊：
影响因子：
2.9
通讯作者：
SCHWAB, ME
中科院分区：
文献类型：
--
作者：
SEILER, M;SCHWAB, ME
碘-125-标记的神经生长因子[NGF]注射到成年大鼠的额叶或背侧前枕叶皮质中的非常小的量，被专门采取并逆行运输到大的，推测胆碱能神经元在基底核区域（外侧视前核，下丘脑前外侧核，无名质，腹侧苍白球和内囊），如光镜放射自显影所示。投射到额叶皮质注射部位的细胞位于同侧的基底核区域的更尾部，而投射到背侧前枕叶皮质的细胞可以在基底核的整个范围内找到，也可以在Broca诊断带的核的垂直和水平分支中找到。其他已知投射到皮质的核团（蓝斑、黑质、中缝核、丘脑）未被标记。与[125 I]NGF相反，注射[125 I]细胞色素C未能通过逆行转运标记基底前脑核团中的任何细胞体。这种高选择性表明神经生长因子或一种密切相关的分子在支配大脑皮层的基底前脑的胆碱能神经元上存在膜受体。
Iodine-125-labeled nerve growth factor [NGF] injected in very small quantities into the frontal or dorsal anterior occipital cortex of adult rats, was specifically taken up and transported retrogradely to large, presumably cholinergic neurons in the nucleus basalis region (lateral preoptic nucleus, anterior lateral hypothalamic nucleus, substantia innominata, ventral globus pallidus and internal capsule), as revealed by light microscopic autoradiography. Cells projecting to the injection site in the frontal cortex were localized ipsilaterally in the more caudal parts of the nucleus basalis region, whereas cells projecting to the dorsal anterior occipital cortex could be found throughout the entire extent of the nucleus basalis and also in the vertical and horizontal limb of the nucleus of the diagnoal band of Broca. Other nuclei known to project to the cortex (locus coeruleus, substantia nigra, nucleus raphe, thalamus) were found to be unlabeled. In contrast to [125I]NGF, injection of [125I]cytochrome C failed to label any cell bodies in the basal forebrain nuclei by retrograde transport. This high selectivity indicates the presence of membrane receptors for NGF or a closely related molecule on these cholinergic neurons of the basal forebrain innervating the cerebral cortex.