Input of Orexin/Hypocretin Neurons Revealed by a Genetically Encoded Tracer in Mice
Input of Orexin/Hypocretin Neurons Revealed by a Genetically Encoded Tracer in Mice
复制标题
DOI：
10.1016/j.neuron.2005.04.029
复制
发表时间：
2005-06
期刊：
影响因子：
16.2
通讯作者：
T. Sakurai;Ruby Nagata;A. Yamanaka;H. Kawamura;N. Tsujino;Yo Muraki;H. Kageyama;S. Kunita;Satoru Takahashi;K. Goto;Y. Koyama;S. Shioda;M. Yanagisawa
中科院分区：
文献类型：
--
作者：
T. Sakurai;Ruby Nagata;A. Yamanaka;H. Kawamura;N. Tsujino;Yo Muraki;H. Kageyama;S. Kunita;Satoru Takahashi;K. Goto;Y. Koyama;S. Shioda;M. Yanagisawa
嗜睡症患者食欲素/下丘脑分泌素缺乏的发现表明，这种下丘脑神经肽在调节睡眠/觉醒状态中起着至关重要的作用。然而，对于产生食欲素/下丘脑分泌素的神经元（食欲素神经元）的突触输入知之甚少。我们应用转基因方法绘制了与食欲素神经元有突触连接的上游神经元群，发现食欲素神经元接受来自几个脑区的输入。这些神经元包括杏仁核、基底前脑胆碱能神经元、视前区gaba能神经元和中缝核/副中缝核的血清素能神经元。由食欲素神经元支配的含有单胺的神经元群没有相互连接，而基底前脑中的胆碱能神经元有相互连接，这可能对巩固清醒很重要。电生理研究表明，碳乙醇刺激了近三分之一的食欲素神经元，抑制了一小部分食欲素神经元。这些神经解剖学的发现为研究调节睡眠/清醒状态的神经通路提供了重要的见解。
The finding of orexin/hypocretin deficiency in narcolepsy patients suggests that this hypothalamic neuropeptide plays a crucial role in regulating sleep/wakefulness states. However, very little is known about the synaptic input of orexin/hypocretin-producing neurons (orexin neurons). We applied a transgenic method to map upstream neuronal populations that have synaptic connections to orexin neurons and revealed that orexin neurons receive input from several brain areas. These include the amygdala, basal forebrain cholinergic neurons, GABAergic neurons in the preoptic area, and serotonergic neurons in the median/paramedian raphe nuclei. Monoamine-containing groups that are innervated by orexin neurons do not receive reciprocal connections, while cholinergic neurons in the basal forebrain have reciprocal connections, which might be important for consolidating wakefulness. Electrophysiological study showed that carbachol excites almost one-third of orexin neurons and inhibits a small population of orexin neurons. These neuroanatomical findings provide important insights into the neural pathways that regulate sleep/wakefulness states.