Complete structures of Bordetella bronchiseptica and Bordetella parapertussis lipopolysaccharides
Complete structures of Bordetella bronchiseptica and Bordetella parapertussis lipopolysaccharides
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DOI：
10.1074/jbc.m513904200
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发表时间：
2006-06-30
影响因子：
4.8
通讯作者：
Vinogradov, Evgeny
中科院分区：
文献类型：
--
作者：
Preston, Andrew;Petersen, Bent O.;Vinogradov, Evgeny
支气管炎波氏杆菌和副肺结核波氏杆菌的脂多糖核心和O抗原的结构是已知的，但这两个区域是如何相互联系的还没有确定。我们对这些微生物的几个菌株的脂多糖进行了研究，以确定脂多糖的完整碳水化合物结构。用不同的化学降解法、核磁共振谱和质谱仪对脂多糖进行了分析。这在所有研究的脂多糖中发现了一个新的五糖片段，它将O链连接到核心。此外，尽管这些细菌的O链被报道为1，4-连接的2，3-diacetamido-2，3-dideoxy-alpha-galacturonic酸的均聚物，但我们发现该聚合物含有几种酰胺化的糖醛酸，其数量因菌株而异。这些新数据描述了两种波尔德氏菌的内毒素碳水化合物骨架的完整结构，并有助于解释这些细菌中内毒素生物合成的复杂遗传学。
The structures of the lipopolysaccharide (LPS) core and O antigen of Bordetella bronchiseptica and Bordetella parapertussis are known, but how these two regions are linked to each other had not been determined. We have studied LPS from several strains of these microorganisms to determine the complete carbohydrate structure of the LPS. LPS was analyzed using different chemical degradations, NMR spectroscopy, and mass spectrometry. This identified a novel pentasaccharide fragment that links the O chain to the core in all the LPS studied. In addition, although the O chain of these bacteria was reported as a homopolymer of 1,4-linked 2,3-diacetamido-2,3-dideoxy-alpha-galacturonic acid, we discovered that the polymer contains several amidated uronic acids, the number of which varies between strains. These new data describe the complete structure of the LPS carbohydrate backbone for both Bordetella species and help to explain the complex genetics of LPS biosynthesis in these bacteria.