Conditioned changes in dopamine oxidation currents in the nucleus accumbens of rats by stimuli paired with self-administration or yoked-administration of d-amphetamine
Conditioned changes in dopamine oxidation currents in the nucleus accumbens of rats by stimuli paired with self-administration or yoked-administration of d-amphetamine
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DOI：
10.1046/j.1460-9568.1998.00125.x
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发表时间：
1998-03-01
影响因子：
3.4
通讯作者：
Phillips, AG
中科院分区：
文献类型：
--
作者：
Di Ciano, P;Blaha, CD;Phillips, AG
在体内的计时电流法被用来监测多巴胺氧化电流的变化，对应于多巴胺流出的大鼠后，提出了一个条件光刺激，反复配对无论是轭或自我管理的静脉注射的精神兴奋剂d-安非他明。每日条件试验开始于非偶然的药物注射，与条件刺激配对，条件刺激由5秒闪光和30秒熄灯组成，之后在3小时期间点亮室内灯，发出药物可用性信号。随后每次注射d-安非他明都与条件刺激配对。在条件试验4-7中进行电化学测量，并且在每个试验中，静脉内d-安非他明（每次注射0.25 mg/kg）自我给药产生平均多巴胺氧化电流的显著最大增加，约高于基线8 nA。多巴胺氧化电流在大鼠接受轭d-安非他明约5 nA以上的基线，第四天的药物管理，并达到约8 nA的第七天和最后一天的药物管理。在第9天的第一次介绍的车辆注射和条件刺激，与照明的房子灯相结合，诱导立即增加在所有的大鼠，以前收到的d-安非他明的脑桥核多巴胺氧化电流。随后介绍的条件刺激在30分钟的间隔诱导细胞外多巴胺氧化电流在两个药物治疗组进一步增加。到3小时会议结束时，两组有类似的最大条件增加多巴胺氧化电流约6 nA。这些数据进行了讨论与药物渴求的神经化学。
In vivo chronoamperometry was used to monitor changes in dopamine oxidation currents corresponding to dopamine efflux in the nucleus accumbens of rats after presentation of a conditioned light stimulus repeatedly paired with either yoked-or self-administered intravenous injections of the psychostimulant d-amphetamine. Daily conditioning trials began with a non-contingent drug injection, paired with a conditioned stimulus consisting of a 5 s flashing light and 30 s lights out, after which a house light was illuminated during the 3 h session, signalling drug availability. Each subsequent injection of d-amphetamine was paired with the conditioned stimulus. Electrochemical measures were taken on conditioning trials 4-7, and on each trial, intravenous d-amphetamine (0.25 mg/kg per injection) self-administration produced a significant maximal increase in mean dopamine oxidation currents of approximate to 8 nA above baseline. Dopamine oxidation currents in rats receiving yoked d-amphetamine were approximate to 5 nA above baseline by the fourth day of drug administration and reached approximate to 8 nA on the seventh and final day of drug administration. On day 9 the first presentation of the vehicle injection and conditioned stimulus, in combination with illumination of the house lights, induced an immediate increase in nucleus accumbens dopamine oxidation currents in all rats that had previously received d-amphetamine. Subsequent presentations of the conditioned stimulus at 30 min intervals induced further increases in extracellular dopamine oxidation currents in both drug-treated groups. By the end of the 3 h session, both groups had similar maximal conditioned increases in dopamine oxidation currents of approximate to 6 nA. These data are discussed with relation to the neurochemistry of drug craving.